Disease Resistance And Mechanism Of The MiRNA Response To Botrytis Cinerea In Solanum Lycopersicum

Botrytis cinerea has become one of the major fruit and vegetable diseases, due to its widehost range and air transmission mode. And Solanum lycopersicum, with the second largestvegetable cultivation, is being seriously affected. B. cinerea cannot be efficiently controlled,and its study from molecular biology focused mostly on phytohormones. MicroRNAs(miRNA) form a class of non-coding RNA genes whose products are small single-strandedRNAs with a length of about22nt, which functions in transcriptional and post-transcriptionalregulation of gene expression. The biosynthesis process of miRNA is complex and it has threekinds of mechanism of action. miRNA function via translational repression or targetdegradation. miRNA play an important role in the interaction between pathogen and plant.The miRNA in S. lycopersicum has been much studied, but the miRNA produced in responseto the B. cinerea is still unclear.Firstly, to screen out the resistance miRNAs, overexpression of pre-miRNAs bothtransiently and stablely was performed. And the expression patterns of those miRNAs werestudied by northern blot and the potential targets were also studied by transient expressionassay.1.According to miRBase annotation, seven pre-miRNAs, which were all differentiallyexpressed were obtained, and were used to constructe the over-expression vector withpBin438vector.2.The resistance of those differently expressed miRNA was tested by transientexpression and four miRNA（sly-MIR482a；ath-MIR319c；ath-MIR394a；ath-MIR172b）which showed good resistance were transformed to Arabidopsis thiana to further studied.3.Tissues pecificity of sly-MIR482a was studied by northern blot and sly-MIR482a wasmore highly expressed in leaves than in roots, stems and flowers.4.The potential targets of miRNA were preliminarily studied.