Monitoring Of Codling Moth And Prokaryotic Expression Vector Construction Of Cydia Pomonella Odorant Receptor Or4

The codling moth,Cydia pomonella (L.), is a key quarantine pests for apple and pear growers and many other fruit crops, which cause the low quality of fruit and premature drop of fruit. In1950s, the codling moth first had found in some areas of XinJiang province, China and then involved rapidly in recent decades. However, in the middle of1980s, codling moth spread from Xinjiang province to Gansu province east via east smoothly then cause a serious loss of fruit industry in both these two provinces. This potential distribution of codling moth not only jeopardizes Xinjiang and Gansu provinces but also endangers whole fruit industry in China. Therefore, in order to avoid losses to fruit plantations, the artificial supervising point is need to investigated the spread direction of codling moth in provinces of Gansu, Shannxi. This study will supervise and investigate codling moth in Gansu and Shannxi province in combination with sex pheromone monitoring method. The results show that codling moth found in Lanzhou area in2013are much more than the ones in2012, which indicates that expansion of codling moth towards east. So far, the trace of codling moth is not found in Shannxi province in2013.Odor receptor existed in membrane protein is a detector to feel chemical stimulation. Functionally, the insect odor receptor act as ligand-gated ion channel and cyclic nucleotide-gated ion channel. This experiment acquired an open-reading fragment (ORF) of codling moth atypical odor receptor4(cpomOR4) through template of cDNA created by reverse-translation and polymerase chain reaction. The results indicated that the insect odor receptors have seven-transmembrane membrane protein with a intracellular C terminus and a extracellular N terminus, with the function of detecting odor compounds. Cloning this gene into pET-32a vector constructed a recombinant plasmid (pET-CpomOR4), which induced by IPTG with a final concentration of lmmol/L for6h. Analyzed by SDS-PAGE electrophoresis, there is a special band at40KD, which is not found in control group. The successful expression of this gene is a key to explore the function of codling moth odor receptor.