Genetic Diversity Revealed By Chloroplast SSR And Cloning Of Transcription Factors NAC1and ABF3of Agilops Tauschii Native To China

Aegilops tauschii Coss.(2n=2x=14, DD) generally is considered as the D genome donor and preciousgenetic resources of hexaploid common wheat. In China, Aegilops tauschii mainly distributed in YellowRiver basin (Shaanxi and Henan provinces) and Yili valley of Xinjiang province, the former belong to theweedy type in the wheat fields, while the latter is a natural wild community. Aegilops tauschii accessions ineach community had formed an independent population and many valuable traits such as strong tillercapability, drought resistance, luxuriant foliage had been detected. Therefore, Chinese Aegilops tauchiiaccessions were potential gene resources for wheat improvement and it is important to explore beneficialgene resources for its further effective utilization. In this paper, to understand genetic diversity and exploresome valuable genes the genetic diversity of32accessions were revealed by chloroplast SSR, moreover,two transcriptional factors NAC and ABF3related to drought stress were cloned from two accessionsXJ002and XJ098which showed the strong and weak drought resistance respectively. The results are asfollows:1. Aegilops tauschii accessions native to China have rich genetic diversity and are valuable geneticresources for wheat improvement.14of18pairs of microsatellite primers in the chloroplast of commonwheat produced clear and stable polymorphic bands and used to assess the genitive diversity of32ChineseAegilops tauschii accessions. a total of44alleles were detected, the average was3.14alleles per primers,the average Nei’s genetic diversity index (He) was0.467. The average polymorphism information content(PIC) value was0.408.2. Cloning and sequence analysis of two transcriptional factors NAC and ABF3in two accessionsXJ002and XJ098showed that there were no differences in the sequences between two accessions. Thecoding region of NAC gene (named as AeNAC1) and ABF3(named as AeABF3) were respectivelyconsisted of996bp and942bp,which can code for a polypeptides with331and313amino acid residues,respectively. Phylogenic analysis showed that AeNAC1and AeABF3had the highest homology respectivelyto OsNAC3subgroup of NAC family group II and AREB/ABF subgroup of bZIP family.