TASA Toxicology And By HPLC Method To Detect Four Kinds Of Alkaloids In Plasma Of Sheep

Three parts were included in this paper:the toxicity test in mice;irritation tests in rabbits and with high performance liquid chromatography to detect four kinds of Alkaloids of Sophora alopecuroides in Sheep plasma. The experimental materials collected in Inner Mongolia Erdos. The main ingredients content were unknown.The first part of the paper was the preface, describes the Sophora alopecuroides growing region and its major active ingredient, summarizes current research focuses on the status of the active ingredients of bitter beans; characteristics and harm livestock major ectoparasitosis.The second part was the experiment section. Including TASA toxicity tests on mice by gavage gets symptoms of acute poisoning, main target organ and recover condition. Bliss method to calculate the median lethal dose of301.75mg·kg-1. Experimental results showed that TASA target organ such as the lung, liver, kidney, heart and nervous system. High doses of TAS A badly damaged lung and the recovery was not satisfactory. Irritation test including TASA skin irritation and eye irritation test. TASA skin irritation included skin irritation test single and multiple irritating irritation test, which were divided into two groups:TASA preparation group and TASA transdermal formulations group. Added transdermal accelerant was azone and propylene glycol. Experimental results showed that low concentrations of TASA showed lower irritating to the skin and eyes.Added transdermal accelerant experimental group showed moderate irritation to the skin, stimulated the stratum corneum hyperplasia and shedding, fiber fracture edema of the dermis and stimulate small vascular congestion, hemorrhage.The reversed-phase high-performance liquid chromatography (HPLC) was developed for determining four kinds of alkaloids SC、SRI、MT and OMT. Acetonitrile was used to deposit proteins in plasma.The eluate was evaporated and dissolved with methanol before detected by HPLC. The mobile phase was organic solvent and Potassium dihydrogen(V/V).The flow was1.0mL/min and the column temperature was main-tained at35℃. Detection wavelength was set at225nm to monitor which was kept for30min. All the calibration curves showed good linearity(R2>0.99) in test ranges. Sophoridine, oxymatrine, matrine and sophocarpine retention time was14.904min,20.641min,28.612min,32.318min, Target peaks well separated. The experiment to establish a method for detecting four major alkaloids of Sophora alopecuroides in sheep plasma. Using non-meridian tube model to calculate the pharmacokinetic parameters. Experiments showed that external used TASAmajor sites of action were body surface and epidermis. Add penetration enhancers also only a very small amount of drug should be into the systemic circulation,which was consistent with their livestock ectoparasites prevention purposes.