| Potato(Solarium tuberosum L.) is the third largest crop in the world. With the development of society and the advancement of technology, In the past, potato breeding focusing on a single high-yielding breeding,now breeding objectives of potato were more and more focused on tuberqualities. Using traditional breeding methods to consume a lot of time andinefficiently, Potato breeding technology will be improved by constructing a high density molecular linkage map and analyzing QTLs of tuber traits.In this study,87tetraploid potato populations and their parents in F1as a test of the material, used for a molecular genetic map construction based on SSR markers, and QTLs analysis of tuber agronomic traits. The main conclusions are as follows:1. Use Joinmap4.0on126pairs of SSR markers for genetic linkage analysis to construct a piece contains13linkage groups, covering11chromosomes, and did not mark positioned on chromosome9, tetraploid potato56SSR markers genetic linkage map. The total length of the pattern is593cm, the coverage is about80%coverage of chromosomes in the genome of2.4cm-92.1cm range of each marker on chromosome number from2to10, the average distance between the two markers was11.3cm. Between the average distance of the largest genetic linkage map for the first seven chromosomes, with an average distance of28.3cm; smallest average distance to the first one chromosome, the average distance of9.2cm. Number of markers on the chromosome is the second largest number of chromosomes, there are10markers on chromosomes, at least the first3,5and8chromosomes were three marks.2. Use MapQTLA.0software, using interval mapping hair, for tetraploid potato yield per plant, number of tubers per plant, tuber rate, dry matter content, starch content, potassium content and other traits QTL mapping studies. QTL controlling potato starch content sites, a total of three:qSC-4, qSC-7and qSC-12were located at four, on the first seven and12chromosomes, LOD value of6.1,3.4and3.2, the contribution of36.1%,7%and12%. Control of potato tuber number per plant sites, a total of QTL2:qTSET-2and qTSET-7were located on chromosomes2and7, LOD value of2.7and3.2, the contribution of31%,36%. QTL controlling potato yield per plant sites, a total of2:qTY-2and qTY-11are located on the first two and four chromosomes, LOD value of2.7and9.1, the contribution is16%,47%. Control of potato tuber rate QTL loci, a total of1:qCPR-2is located on chromosome2, LOD value of5.7, the contribution is11%. QTL controlling potato dry matter content sites, a total of2:qDMC-1-1and qDMC-1-2are located on the first chromosome, LOD value of5.1and7.0, the contribution of36%,13%. QTL controlling potato potassium content sites, a total of1:qPC-2. qPC-2is located on the second jump chromosome, LOD value of4.0, the contribution of8.5%.3. All11QTL loci obtained in this study are new tetraploid potato QTL sites, some of their predecessors diploid potato QTL mapping with the same or nearby place. Atlas finesse only be used in this experiment produced a preliminary coarse positioning, but also to the subsequent fine positioning and orientation of previous work certification provides reliable evidence... |
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