Studies On Tissue Culture And Rapid Propagation Technology Of Lonicera Edulis

Lonicera caerulea L. var. edulis Turcz. ex Herd, also known as Lonicera edulis, black bear, Yama Ko etc., is an edible berries with world rare, pure natural, green and wild, with high medicinal value and commercial value, known as "The third generation fruit tree’", the fruit which is rich in sugars, organic acids, minerals, vitamins and a variety of trace elements. Lonicera edulis study was mainly focused on nutritional composition and pharmacological aspects both at home and abroad, it was not deep in artificial cultivation of fine varieties and wild resources depleted. In this paper, combining the tissue culture and rapid propagation with cultivate new varieties of Lonicera edulis, conduct systematic research, use the dormant branches generation of axillary buds as explants, screening of explants disinfection method for tissue culture, the purpose is to explore effective ways of isolated culture of Lonicera edulis, to establish a rapid and efficient breeding system, to provide theoretical casis and technical support with improve seeding breeding efficiency, accelerate the breeding process, protect and rational utilization of Lonicera edulis, greatly improve economic value and development value aspects. The main results as follows:1.After explant pretreatment, soaking for15-20s by75%ethanol, sterile water to wash3times, then treatment for15-20s by0.1%HgCl2, sterile water to wash3times, contamination rate less than6.33%, death rate less then8%, germination rate up to85.67%.2.Effects of different kinds and concentrations of exogenous hormones and basic culture on primary culture of Lonicera edulis:Using the hormone of6-BA, IBA and GA3alone could induce the differentiation of axillary bud, effect of differentiation by2.0mg·L-16-BA was the best, differentiation rate reached92%, differentiation rate reached85.33%by0.3mg·L-1IBA, differentiation rate reached84.00%by2.0mg·L-1GA3, use the basic culture alone could induce the differentiation of axillary bud, the differentiation rate of MS was the highest, up to93.33%. Different kinds and concentrations of hormones and basic culture of the orthogonal results show, differentiation rate was the highest in MS+6-BA2.0mg·L-1+IBA0.3mg·L-1+GA31.5mg·L-1,up to95.92%.3.Inoculation in the best differentiation culture, effect of differentiation for30d was better, average height was8.43cm, average dry weight reached0.018g, average leaf area reached0.14cm2, average differentiation rate reached97.85%.4.In the subculture proliferation process, the results of orthogonal test showed that different kinds and concentration of hormone and basic culture, the multiplication coefficient reached4.14in MS+6-BA2.0mg·L-1+IBA0.3mg·L-1+GA31.5mg·-1, subculture cycle for30d was appropriate, seedling growth robust, the multiplication coefficient was4.20.5. Effects of different kinds and concentrations of exogenous hormones, active carbon and basic culture on rooting culture of Lonicera edulis:Using the hormone of NAA and IBA could induce the seedings rooting, effect of rooting by0.2mg·L-1NAA was the best, rooting rate reached90.67%, the rooting rate reached92%When the concentration of activated carbon was0.1%, use basic culture along, the effect of rooting in MS was the best, up to89.33%. Different kinds and concentrations of hormones, active carbon and basic culture of the orthogonal results show, the rooting rate was the best in MS+NAA0.2mg·L-1+activated carbonl%, up to98.00%.6. Inoculation in the best rooting culture, effect of rooting for18-24d was the best, average height was8.43cm, average root lengh was3.78cm, average root number was10, average rooting rate was99.33%.7.In the acclimatization and transplangting of seeding process, the survival rate of acclimatization for2d was the best, up to92.67%, the most suitable transplangting substance was mountain soil:moss=1:1, survival rate up to94.00%.