| Tomatoe is one of the very obvious heterosis vegetable crops.The use of male sterile line to produce hybrid seeds in tomato can save a lot of manpower and resources, reduce seed costs and ensure seeds purity. Due to the fertile pollen, the ps functional male sterile line can be achieved to save the sterile traits by artificial pollination, and its sterile traits are stable.So it will have great value and potential in breeding and scientific research in future. In order to further explore the mechanism of ps male sterile line so that it can be more fully applied in the production of hybrid seeds, we used the SuperBSA method to make the ps gene to be fine mapped, combined the paraffin section method with scanning electron microscopy method to observe the sterile traits and maked use of the high performance liquid chromatography to measure the endogenous hormone content of anthers, the main results were obtained as following:1. The early developments of infertile and fertile anthers were basically similar. Until the flowerbud grew up to0.8cm,a series of trichome cells had been differented from the back of the sterile anthers and bonded with the inside cells of the petal main vein.Though there were less differentiated trichome cells at the back of fertile anthers, but were not bonded with the petal main vein.We also observed that when the petals opening angle of60°, compared with the fertile flowerbuds, the petals and anthers of sterile flowerbuds sticked together, the petals didn’t open, the anthers were bound by the corolla, and anthers dehiscence were blocked. Meanwhile the front-end cells of the connectivum in sterile anthers didn’t degrade and disconnect with the anther wall just like the fertile anthers’s,this will to some extent prevent pollen out.The anther wall of sterile anthers was thinner,and the two same side rooms of sterile anthers didn’t connect together.2. In this study, we used the scanning electron microscopy method to observe the condition of anther dehiscence in sterile anthers and found that in the petals opening angle of60°, the fertile anthers already had a certain degree of cracking.We could see pollens in the upcoming gap, and the anther wall cells arranged regularly and closely. Although there are cracks in the sterile anthers, but cracked extent significantly less than the fertile anthers, and at the dehiscence gap of sterile anthers we found some degraded cellular debris. These fragments compactly distributed in the cracks, and prevented the pollen spilling out. In the observation of the mature anthers we found that mature sterile anthers although will rarely crack and loose pollens, but the released pollen always sticked together, and some pollens had shriveled and lost vitality. But the pollens of mature fertile anthers almost didn’t lose vitality.3.The ZT content was more in sterile anthers than fertile anthers before the flowerbuds0.8cm long, and less than fertile anthers after0.8cm.The GA3content of sterile anthers and fertile anthers almost presented the same trend, the difference was not obvious.The ABA content had no obvious difference between sterile anthers and fertile anthers before flowers opening30°angle, and was less in sterile anthers than fertile anthers after that, but in bloom time it was higher than sterile anthers. The IAA content in sterile anthers had no obvious changes before the bud0.8cm length,but after that it increased obviously and higher than fertile anthers.and then slightly less than fertile anthers when flowers opening45°and60°angle, and finally obviously higher than fertile anthers when flowers opening90°angle.The variation trend of endogenous salicylic acid content in sterile anthers and fertile anthers were precisely opposite.4. There were84,630SLAF markers totally had been developed in this experiment.The overall average depth of the markers reached249.33x, so we simplified the genome successfully.1,983polymorphic markers were obtained by analyzing the olymorphism of samples. We got5specific markers by correlation analysis, and the ps gene was successfully located in a candidate region on chromosome2of tomato. The size of the candidate region was0.8497Mb, and there were a total of104genes in the region, and finaly the104genes had been annotated. |
