The Study Of Two Watermelon Varieties In The Process Of The Development Of Lycopene Accumulation And Related Enzyme Gene Expression

Lycopene is an intermediate product of carotenoid biosynthesis, isoprenoids metabolism synthesis by.Watermelon varieties between lycopene content difference is very big, and different crumb color watermelon in lycopene, which is not the same.Lycopene in the process of the synthesis of enzymes involved in eight main hydrogen lycopene synthetase (Psy), eight hydrogen lycopene dehydrogenase (Pds), zeta carotene dehydrogenase, lycopene cyclase (Lcyb), etc.Lycopene content is not only related to genotypes, and by various enzymes in watermelon throughout the development process of regulation.Therefore understand the watermelon to the entire development process of lycopene accumulation and related enzyme gene expression characteristics to raise the content of the watermelon lycopene has very important significance.This test to a watermelon strain LSW-177(red) and the "Cream" of Saskatchewan (weak yellow flesh) as materials, optimization of the high performance liquid chromatography (HPLC) determination of lycopene conditions, determination of the two varieties from flushing to overmature period of7different time points watermelon pulp, lycopene content in the corresponding period determined by fluorescence quantitative PCR related enzyme gene (Psy, Pds, Zds, Lcyb) expression of the quantity change, has been clear about the watermelon lycopene accumulation of key enzyme genes, at the same time, compared Lcyb gene sequence differences between the two varieties, eventually to take effective technical means, improve the watermelon in lycopene, which is to lay the foundation.This test to get the following main conclusions:1, optimization of the high performance liquid chromatography (HPLC) determination of lycopene conditions:choose methanol, acetonitrile-methylene chloride (2:5:3) as mobile phase;The flow rate of1.0ml/min;Detection wavelength of472nm;Column Is the room temperature;Sample quantity for10mu1.Used reagent and ultrapure water after0.2mu m before using the membrane filter, and ultrasonic cleaning for30min.2, red watermelon16days after pollination, lycopene began to accumulate, around the fruit color period (24days after pollination) rapid accumulation of lycopene, the mature period (40days after pollination) peak, then decreased.And in the entire development process of weak yellow flesh watermelon had been detected at the accumulation of lycopene.3, four related enzyme gene (Psy, Pds, Zds, Lcyb) express content there are differences between the two varieties.Lycopene accumulation and Psy was significantly positively related to gene expression, gene expression was significantly negative correlation with Lcyb.Lcyb and combining Psy genes expression differences between the two varieties, especially in pulp turn color difference analysis, and speculated that Psy gene and Lcyb gene is the key to the watermelon form lycopene.4, between the two varieties of lycopene cyclase Lcyb genetic test results show that there are three SNPS loci and two protein change between the two varieties.