A Study On Anti-oxidative Ability Of Sorghum Dochna Under Water Stress

Energy is an important factors for the development of the current global economic. Most of theconventional energy is non-renewable, with the non-renewable resources depleting, developing renewableenergy, protecting energy and ecological security have become the focus of future development. Bioethanolhas been the best way to replace fossil energy sources because of renewable and CO2emissions neutral.Using aging grain such as Corn and wheat to produce fuel ethanol, Will cause conflicts compete for foodbetween Vehicle and humans. Ensure food security, accelerate the improvement of the energy consumptionstructure, increase the proportion of renewable energy is the future direction of development. Sorghumdochnahas a great advantage who could replace non-renewable energy. Our country has nearly100millionhm2marginal land isn’t suitable for cultivated farmland species. This part of the land has low productivity,dry and barren. The use of marginal land to develop energy plant cultivation, not only can form a stableEnergy plants supply base, but also improve the structure and quality of the soil, to expand the utilization ofarable land. To a certain extent reduce planting land shortage tensions and ease the energy crisis.Moderate water deficit maked crops produce adapting reactions and optimal choice, but different croptypes, species and growth stages exist difference in drought tolerance. In this experiment, Sorghum dochnaas the experimental material, by controlling the different levels of water stress and rewatering treatmentafter drought, researched the adaptability responses of Sorghum dochna to water deficit under differentdrought stress and in different growth stages; measured and analyzed the antioxidant enzyme activities ofSorghum dochna and studyed the quantitative expression of the main antioxidant enzymesgenes in order tofind out the feedback regulation mechanism of Sorghum dochna under adversity. Try to find out the mainregulatory periods of the main antioxidant enzymes of Sorghum dochna and the critical irrigation periodwhich can significantly improve the leaf water use efficiency.The results are as follows：1. Under different levels of water stress and rewatering treatment, Leaf water use efficiency ofSorghum dochna were higher than Sorghum bicolor. Leaf water use efficiency of Sorghum dochna werehighest during filling-booting stages, It showed that Sorghum dochna are sensitived for water stress, andmade the positive physiological response. Speculated the best irrigation period of Sorghum dochna mightbe in the filling stage.2. Under different levels of water stress and rewatering treatment, SOD activity of Sorghum dochnawas higher than Sorghum bicolor in joining-booting stageand filling stages. rewatering treatment three daysand six days later, SOD activity of Sorghum dochna was decline, slightly lower than Sorghum bicolor.Under rewatering treatment, SOD activity of Sorghum dochna recovery speed more quickly in jointingstages,The POD activity change trend was not obvious during three periods. Under lasted water stress,SOD activity of Sorghum dochna was higher than Sorghum bicolor, the high margin was following theplants growing stage increasing.POD activity of Sorghum dochna were higher than Sorghum bicolor under the last water stress inseeding stage and joining-booting stage. Indicated that SOD has the strongregulation ability to Sorghum dochna in joining-booting and filling stages. The major role of POD inseedling stage.3.Drought can induce the expression of antioxidant enzyme SOD, POD genes. The expression of PODgene in Sorghum dochna showed that first decreasing and then increasing thoughout the whole stage. Aslight change in the expression of POD gene, but on the whole expression of the trend, the changesfluctuate within negligible limits.POD genes in the regulation of water stress environment is not obvious;Differences in gene expression of SOD gene in the whole growth period is obvious, the highest expressionin joining-booting stage. Presumably that for Sorghum dochna under water stress, antioxidant enzymesSOD gene mainly regulation adaptation mechanism.4.Planting in arid, semi-arid environmental conditions, Sorghum dochna has obvious advantage,Sorghum dochna could be widely planted in marginal lands. But the response of plants to water stress isavery complex process, which includes a variety of co-regulated genes. Further confirmed that antioxidantenzymes SOD gene is the main function of Sorghum dochna under arid environment. Need to conduct amore in-depth study.