Study Of The HDAC1Gene Snp And Its Correlation With Partical Economic Traits In Cattle

Several studies revealed that there were close relationship between histone deacetylase (HDACs) and gene transcriptional activity. Nucleosome histones in transcriptional active region are highly acetylated, whereas those in inactive region are in the state of de-acetylation. HDAC plays an essential part in gene transcription, signal transduction and development, cell multiplication and growth.Adopting Polymerase Chain Reaction-Single Strand Conformational Polymorphism (PCR-SSCP) and DNA PCR production sequencing, and HDAC1gene used as candidate of economic characters for Nanyang cattle and Simmental cattle, the single nucleotide polymorphism of gene order in HDAC1gene3’UTR, Intron4-Intron5, Intron10-Intron11was analyzed in15Nanyang cattle and37Simmental cattle, and the relevance between the SNP and the economic characters in Nanyang cattle and Simmental cattle were also detected. The purpose is try to improve and utilize Nanyang cattle and Simmental cattle, as well as to breed their new varieties and improved varieties, accelerate to breed good cattle. The main experiment results were summarized in following:1.250bp fragment in Intron4-Intron5,276bp fragment in Intron10-Intron11and368bp fragment in3’UTR of HDAC1in cattle were obtained, respectively.2. There were no polymorphisms in Intron4-Intron5locus of HDAC1in Nanyang cattle and Simmental cattle by PCR-SSCP and the direct sequencing analysis.3. There was a mutation locus in Intron10-Intron11of HDAC1in Nanyang and Simmental cattle, all of them have a mutation of A/G locus, which belonged to synonymous mutation. On the other hand, the homozygote GG frequencies were the highest, while homozygote AA frequencies were the lowest in Nan yang cattle. Heterozygosity AG frequencies were higher than homozygote frequencies in Simmental cattle. Allele G took the most parts in both cattle. The results of correlation between SNP and the economic characters showed that the waist angle width in AG genotype Nanyang cattle was longer than that of in AA genotype (P<0.05), and the dominant effects of this locus were significant. The backfat thickness in AA genotype Simmental cattle were lower than that of in GG genotype and AG genotype individual, and the different was significant (P<0.05), the gene additive effects of this mutant locus on backfat thickness were significant (P<0.05)4. There were two mutations in3’non-translation region in HDAC gene of Nanyang cattle:the SNP of A/C locus in50bp site showed the frequencies of AA genotype were larger than that of AC genotype; the SNP of T/C locus in199bp site showed the frequencies of TT genotype were larger than that of TC genotype. A nucleoside allele took80%in both SNPs. There were no SNP in3’non-translation region in HDAC1gene of Simmental cattle.5. Results of relevance analysis of A/C locus in3’non-translational region of HDAC showed that the mean body length, body height and hip cross in AC genotype group were significantly higher than that of in AA genotype ones (P<0.05). There were no significant influences of T/C locus in Nanyang cattle on its growth traits.In conclusion, several SNPs were found in HDAC1gene, some loci had effects on growth and slaughtering traits of cattle, and they could used as potential molecular markers in cattle breeding.