Isolation,Identification And Establishment Of Yanbian Bears PCR Diagnostic Methods Streptococcus

Streptococcal disease (Streptococosis) is mainly a variety of Zoonosis general term by the (β-hemolytic streptococcus caused. Animal streptococcal disease mainly in pigs, cattle, sheep, horses, chickens are more common. In recent years, mink, yak, rabbit and fish have also been reported occurred streptococcal disease. People with scarlet fever streptococcal disease is more common. Clinical diagnosis of streptococcal disease varied manifestations, can cause a variety of suppurative invasive and sepsis, but also showed the limitations of infection. Streptococcus species range, is widely distributed in nature, mainly transmitted through the respiratory tract and the air, followed by skin lesions can also spread the infection, the main cause of lobar pneumonia, bronchitis, meningitis, septic arthritis, sepsis etc., can produce a variety of toxins and enzymes outer dissolve red blood cells, the lethal effect on the animals. Data shows that when these diseases caused by Streptococcus occur, can infect humans, livestock, flowers whales, foxes and other wild animals, a few days after the birth of an infant or young animals most of meningitis due to higher mortality rates, resulting in incalculable economic losses.In recent years, the gradual expansion of streptococcal disease in pigs and other animals in the popular area of Yanbian, morbidity and infection rates continue to rise, today has become one of the important livestock disease a serious threat to the development of Yanbian, therefore, the separation of Streptococcus local strains established detection methods and vaccine research is necessary. Isolation and identification of Streptococcus Yanbian to bear strain, as well as the prevention of Streptococcus bear in the area to support the popular industry, the use of morbidity and mortality trials Bear diseased successfully isolated three strains of pathogens, and applied testing H2O2, Austria spectrum experiments, bile lysis experiments to identify the biochemical reactions and biochemical characteristics of this three strains, as well as Gram staining and molecular biology diagnostic AMOS-PCR conducted a preliminary identification. The results showed that the improvement in the isolated blood agar colonies specificity; strains isolated in H2O2 bear no bubbles test reaction, bile lysozyme experimental biochemical results were negative, Gram staining clearly visible Gram-positive spherical bacteria AMOS-PCR showed that the strain has760bp and the specificity of PCR diagnostic methods in598bp created two clear bands, these results prove that the initial separation of streptococcus. bacteria.16SrRNA bacterial identification based on universal primers to total DNA extracted streptococcal isolates was amplified by PCR as a template specific target gene, cloned into pMD18-T vector, sequenced delivery of biological agents, to compare the NCBI, compliance with Streptococcus99.8%selected gene sequence in a gene on this design primers, PCR for diagnostic methods, PCR amplification of this bacterium, the same cloned into18-T vector, sequenced delivery biotech company the sequencing results with the original gene sequence alignment was100%in the NCBI to compare with the original matching results. This test provides a theoretical basis for the further development of diagnostic methods bear streptococcal disease, but also for the prevention and treatment of disease bears lay the clinical foundation.