Effect Of Selenium Deficiency On Chicken Thyroid And Kidney Function

Selenium is one of the essential trace elements for animals, it has many biological functions, such as antitumous effect, detoxification, antioxidation, regulates the metabolism of thyroid, enhance immune function and prevents diseases etc. Which makes the biological function mechanism of selenium are widely recognized, and selenoprotein has become a hot spot of research on the world in recent years.Selenium deficiency disease is a kind of common nutritional metabolic disease in Poultry production, it showed necrosis of the muscle and pancreas, exudative diathesis and pathological changes. At present, many reports on selenium deficiency could lead to histological damage of pancreas and digestive tract, but the damage of kidney and thyroid tissue induced by selenium deficiency had rarely reported.In order to explore the damage mechanism of selenium deficiency on kidney and thyroid, our experiment copy the selenium deficiency disease model of chicken, we detect the expression of inflammation genes HO-1and iNOS mRNA and protein in kidney,25selenoproteins in thyroid and the content of thyroid hormone in plasma, and the antioxidant function of kidney and thyroid tissue in chicken. The results showed that:1. In the trial period, there are some symptoms such as diarrhea, exudative diathesis in chicken of selenium deficiency group, the results show that selenium deficiency could induce the damage of organization structure in chicken, we successfully reproduced the selenium deficiency model.2. Fusion proteins of HO-1and iNOS were expressed in prokaryotic system. The polyclonal antibodies were prepared by using expressed fusion proteins.3. The expression of iNOS and HO-1mRNA and protein in kidney are increased by selenium deficiency, uric acid and creatinine are common index of renal function test, selenium deficiency increased the content of creatinine and uric in plasma, and decreased the antioxidant function of kidney in chicken.4. Thyroid hormones are important endocrine hormones, and it played many important biological functions in the chicken. Selenium deficiency decreased the content of Thyroidal Triiodothyronine (T3) and Free Thyroidal Triiodothyronine (FT3),and increased Thyroidal Thyroxine (T4), Free Thyroidal Thyroxine (FT4) and TSH, and decreased the antioxidant function of thyroid in chicken.5. Selenium deficiency could influence the mRNA expression of25-selenoprotein in thyroid of chicken. Application of principal component analysis showed that mRNA expression level of eight selenoproteins (Diol, Dio2, Dio3, Txnrd2, Seli, Selu, Gpxl and Gpx2) had a similar downward tendency.The results above showed that, selenium deficiency can induce hypothyroidism, oxidative stress of thyroid and kidney tissue, this is one of damage mechanism of selenium deficiency on the thyroid and kidney tissue.