| Litopenaeus Vannamei is an ideal culture varieties，along with the rapid developmentof the shrimp farming industry and the rising culture level, pollution problems ofshrimp farming can not be ignored. Bioremediation has a broad developmentprospects for low cost, effective, non-polluting.This research will add bagasse thatafter different pretreatment and Bacillus to shrimp higher-place pond，to performculture experiments. By studying the growth of shrimp, water quality factor (TAN,NO2-N, NO3-N, TN, PO43-, Chl.a, TSS, TA) bacterial content (Bacillus,Vibrio,heterotrophic bacteria), explore the effect of each pretreatment,in order to providestheoretical support for the use of bagasse in shrimp culture.The main contents are as follows:1. Study on pretreatment effects of bagasseWeight of bagasse decrease44.0%after NaOH treatment, decrease26.0%afterSulfuric acid treatment, and the control group weight loss12.1%, NaOH treatmentgroup’s weight decrease rate were significantly higher (P <0.05) than the controlgroup. About13%bagasse can pass100mesh sieve before dealed with bacteria.100mesh sieve pass rate of each group was increase with the processing time, after48hthe S5group pass rate reached48.3%, mixed group reached46.2%, better than BZ5group(38.2%) and the control group(36.3%).2. Application of crushed bagasse in Litopenaeus Vannamei cultureBy comparing the bagasse group (adding crushed bagasse and Bacillus) and thecontrol group shrimp growth,changes of water quality and bacterial content, analysisof the effect of adding bagasse. The results showed that, the yield and survival rate ofbagasse group were15.6%and7.3%higher than the control group, shrimp weight ofbagasse group is12.0%heavier than the control group.In the late phase, TANmaximum concentration of bagasse group is higher than the control group, up to1.74mg/L. At the end of cultivation, each group’s NO2-N concentration reaches a maximum concentration, bagasse group’s concentration is1.34mg/L,higher than thecontrol group. Bagasse group’s TN maximum concentration is4.95mg/L, higher thanthe control group. Bagasse group’s PO43-and TSS totality content were higher than thecontrol group’s, and Chl.a totality content is lower than the control group’s. Bagassegroup’s Bacillus density of water and shrimp intestinal were significantly higher thanin the control group(P <0.05).In the middle phase,Bagasse group’s shrimp intestinalheterotrophic bacteria density is higher than in the control group. Overall, add crushedbagasse to the ponds is help to improve the yield and survival; make little effect towater quality; can obviously improve the growth of bacteria.3. Application of soaked bagasse in Litopenaeus Vannamei cultureIn this experiment, bagasse after seawater containing Bacillus soaking7d and21d.By comparing the bagasse group A (bagasse soaking for7d and Bacillus), the bagassegroup B (bagasse soaking for21d and Bacillus) the control group shrimpgrowth,changes of water quality and bacterial content, analysis of the effect ofadding soaked bagasse.The result show, the yield and survival rate of experimentalgroups were lower than the control group, but not significantly (P>0.05), yield andsurvival rate of bagasse Group A were the lowest. In the late phase,TAN maximumconcentration of the experimental groups were lower than the control group, bagassegroup A TAN concentration is0.138mg/L which is lowest, significantly lower(P <0.05) than the control group. In the late phase,NO2-N concentration in theexperimental group were significantly lower (P <0.05) than the control group, thelowest concentration was0.026mg/L in bagasse Group A.Chl.a content was instableduring culture.At the end of the culture, bagasse group A ’s concentration (162.3μg/L)was the highest, the control group was the lowest (84.0μg/L).At the end of culture,bagasse Group B’s TSS content (100.2mg/L)was highest, the control group’s(83.4mg/L)was the lowest.At the end of farming, experimental groups’ Bacillus inthe water and was significantly higher (P <0.05)than the the control group’s. In themiddle of culture, the cultivable heterotrophic bacteria concentration of group B wassignificantly higher (P <0.05) than the control group. In the late phase,intestinalbacillus concentration of the experimental groups were significantly higher (P <0.05) than the control group. Vibrio and culturable heterotrophic bacteria of theexperimental groups were higher than the content of the control group. Overall, addsoaked bagasse will definitely help to improve water quality, reduce ammonia andnitrite nitrogen, will increase the water content of Total Suspended Solids.Canpromote the growth of bacillus and shrimp, heterotrophic bacteria, adding by21dtreated bagasse is more effective, add7d treated bagasse can help the changing ofbacteria content become more stable. |
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