The Preliminary Study Of WSSV Carrying, Immune Related Enzyme And Disease Resistance Of Shrimp Cultivated With Gammarus

1．The survey of the carrying amount in Food organisms and shrimpcultivated in pondssample the Food organisms and Penaeus japonicas in the pond cultivate thePenaeus japonicas from the places called Diao Kou, Nan Yuan, Hai Yang. Resultshows that: In Diao Kou, the carrying amount of WSSV in Gammarus was firstincreased and then decreased. The highest carrying amount was105level and wassignificantly higher than the others, the carrying amount of WSSV in other time wasin104level. The carrying amount of WSSV in CoroPhium was grow slowly, themount was in104level. The The carrying amount of WSSV in Potamocorbularubromuscula has no significantly tendency, the amount of WSSV was in104level.The carrying amount of WSSV in Artemia and Nereidae was in106and104levelrespectively. In Nan Yuan, the carrying amount in Gammarus was first decreasedand then increased, but the amount was not higher than104level. The carryingamount of WSSV in CoroPhium has the tendency increase and the carrying amount ofWSSV was in104to105level. The amount of WSSV in Potamocorbularubromuscula has no significantly tendency. The amount of WSSV was in104to105level. The carrying amount in Penaeus japonicas was in105level. In Hai Yang, thecarrying amount in Gammarus was first decreased and then increased and the carryingamount of WSSV was in103to105level. The carrying amount of WSSV inCoroPhium has the tendency increase and the carrying amount was in104level. Theamount of WSSV in Potamocorbula rubromuscula has no significantly tendency andthe carrying mount was in103to105level. The carrying amount in Penaeusjaponicas was in105level and that was higher than the carrying amount in Penaeusjaponicas in other places. The amount of WSSV in Assiminea violacea was in104level. The amount of WSSV in Fresh fish Miscellaneous Was in105level. 2、 The explore on Litopenaeus vannamei’s ability to prey onGammarusLitopenaeus vannamei has the ability to prey on Gammarus. Litopenaeusvannamei with the body length from4cm to7cm can prey on Gammarus in almost allthe size, Gammarus in the size from12mm-17mm can not be preied by Litopenaeusvannamei with the body length in4cm. The ability to prey on Gammarus wasenhanced with Litopenaeus vannamei’s body length growth. The ability to prey onGammarus was significantly related to the length of pereopod. Litopenaeusvannamei with longer body length is tend to prey larger Gammarus. But,Litopenaeus vannamei can’t eat their fill only by Gammarus, it is necessary to feedsome feed in the process of breeding3、 The influence of feeding small litopenaeus vannamei withGammarus on growth,the activity of immune related enzyme and thedisease resistanceThree treatments was set to study the effect of difference feeding pattern on smalllitopenaeus vannamei’s growth and the active of immune-related enzymesb,Gammarus treatments, Alternating treatment and Feed treatment. The resultsshowed that, Feeding Gammarus can promote the growth of small litopenaeusvannamei. The WGR of Gammarus group and Alternating group was improved21.2%and17.6%respectively compared with Feed group. Feeding Gammarus cansignificantly improve the survival rate of small litopenaeus vannamei. The survivalrate of Gammarus group, Gammarus group is65%、66%、57.3%respectively. FeedingGammarus can significantly improve the activity of phenoloxidase in Alternatinggroup, can also significantly improve the activity of SOD. The order of activity ofSOD(from high to low)is Gammarus group, Alternating group and Feed group.Feeding Gammarus can improve virus resistance of small litopenaeus vannamei.The time of Semilethal of Gammarus group, Alternating group and Feed group is112h、90h、72h respectively. Overall, feeding Gammarus can promote growth can improve the activity of some immune enzyme and virus resistance of smalllitopenaeus vannamei.4The influence of feeding lager litopenaeus vannamei withGammarus on growth,the activity of immune related enzyme and thedisease resistanceThree treatments was set to study the effect of difference feeding pattern onlitopenaeus vannamei’s growth and the active of immune-related enzymesb,Gammarus treatments, Alternating treatment and Feed treatment. The resultsshowed that, the survival rate of Gammarus treatment, Alternating treatment wassignificantly higher than Feed treatment. There was no significant difference amongthree treatments. There excited on significant difference in specific growth rate.50days later, POD, SOD, PO, antibacterial and muramidase were higher than those inAlternating treatment and Feed treatment. The Feed treatment was highest in thecarrying volume of WSSV, Alternating group was lowest, there was significantdifference among three treatments. Attacked by WSSV, the half lethal time inGammarus group, Alternating treatment were prolonged by9.52%,19.05%comparedwith Feed treatment. Comprehensive analysis, feed Litopenaeus vannamei canimprove the activity of immune related enzymes, can improve the survival rate anddisease resistance.