| Shanlan Upland Rice is an important resource of upland rice in Hainan province. Shanlan Upland Rice has some excellent characteristics including drought resistance and barren resistance. Early reports concentrate on germplasm resources of Shanlan Upland Rice and few studies on the drought assessment and drought resistant mechanism. The research involving in clarifying the drought resistance mechanism of Shanlan Upland Rice has many guiding significance to generate new varieties of drought resistant crops.Protein is the function actor of organism. Compared with the gene level, proteomics can describe the change and the adaption of plant more intuitively under adversity stresses. Proteomics has been more and more widely used in stress-resistant research in plants.In this study, Shanlan Upland Rice was used as experimental materials. To estimate the drought resistance ability of Shanlan Upland Rice and determine the protein expression of Shanlan Upland Rice under drought stress. We design the normal water supply treatment drought and water treatment, dry treatment three treatment groups and measure their morphological index, physiology index and protein expression. The results are following:1. By measuring the morphological index(including plant height measurement, leaf area measurement and root length measurement) and physiology index(including determination of proline content, chlorophyll content, MDA content) of Shanlan1764,3#Baisha, Gangyou527and9311under dry treatment or normal water supply treatment. This research points out that the drought resistance ability of Shanlan Upland Rice is obviously better than9311and less than Gangyou527.2. Using the TCA-acetone method to extract the leaf protein of Shanlan1764under different treatment (normal water supply treatment, drought and water after dry treatment). Two-dimensional polyacrylamide gel electrophoresis is used to separate protein spot. Image Master2D Platinum shows that there are137protein spots significantly altered including71proteins up-regulated and66proteins down-regulated. Mass spectrometry analysis for45protein spots and28proteins are identified. According to the protein function,28proteins can be divided into five types:(1) glucose metabolism related proteins;(2) energy metabolism related proteins;(3) amino acid and protein synthesis related proteins;(4) photosynthesis related proteins;(5) other proteins.In summary, this study determined the Shanlan Upland Rice does have more excellent drought resistance capability. The proteomics analysis of Shanlan Upland Rice provide the data support on the level of molecular biology and provides an important reference to subsequent research related gene and protein regulation. |
