Study On Phenotypic Diversity Of Natural Population In Acer Grosseri Pax

Acer grosseri Pax. also known as green husk belonging toAceraceae,has important ornamental value,medicinal value andeconomic value.in order to better use and protection of A.grosseri,form two aspects of phenotypic and molecular study of geneticdiversity in A.grosseri,reveals the variation and variationregularity.The main results were as follows:1.By34phenotypic traits of24natural populations were studied inA.grosseri.The results showed:(1)Variance analysis of phenotypictraits of leaves,fruits and seeds showed that there were extremelysignificant differences among and within populations except forKFLW、FL、FW and FLW.(2)The average coefficient of variation (CV) of24traits was18.19%，The order of CV was seed（13.11%）<fruit（14.79%）<leaf（21.23%），the trait of seed was better stable.(3)Themean phenotypic differentiation coefficient (VST) was52.2216%,the variation among populations(52.2216%) was approximately equal tothat within populations(47.7784%).(4)Shannon-Wiener informationindex of populations was1.7700,the order Shannon-Wienerinformation index of population belonging to mountain is yanshanmountain<taiyue mountain<zhongtiao mountain<qinling mountain<taihang mountain<funiu mountain<tongbai mountain<dabiemountain.(5)The phenotypic variation appeared gradient regularchanges,The variation of leaf affected mainly by longitude andlatitude in spatial distribution,fruit and seed gradually becomesmaller from south to north,the trend of gradually smaller with theincrease of altitude.(6) The populations of A.grosseri investigatedcould be divided into two groups according to the UPGMA clusteranalysis.which clustering depends on the geographical distance,usedMantel’s test for geographic distance and euclidean distance analysisof the correlation(r=0.198),but the inspection was not significant.2.Genetic diversity and genetic structure was studied using SRAPmarkers on360materials of24populations in A.grosseri.The resultsshowed:(1)140DNA bands were generated in11primers and115ofthem were polymorphic bands, which was82.14%of the total bands.Each primer pair generated10polymorphic bands on average and themean polymorphism rate was80.49%.(2)the mean observed numberof alleles (Na) was1.7781, the mean effective number of alleles (Ne) was1.6126, the average Nei’s gene diversity index (H) was0.3472,the total Shannon’s index (I) was0.4976,the average percentage ofpolymorphic loci was77.81%, the change was more consistent in thevariation of Na、Ne、H、I and PPB.(3)The total genetic diversity Hspwas0.4396in24populations,genetic diversity Hpopwithin populations was0.3595,genetic variation within populations accounted for81.45%ofthe total genetic variation and genetic variation between populationsfor only18.55%;The average Gst was0.1964, the variation occurredbetween populations accounted for19.64%,and80.36%occurredwithin populations; genetic variation between populations accountedfor10%and within populations accounted for90%using AMOVAanalysis;which illustrated genetic variation within populations isgreater than between populations.gene flow Nm was form1.7261to7.1938,with the average was3.4223,gene exchange present in thepopulation of A. grosseri.(4)the observed number of alleles (Na)、theeffective number of alleles (Ne)、the Nei’s gene diversity index (H)、the Shannon’s index (I) and the percentage of polymorphic loci (PPB)were significant negative correlation with latitude,genetic variationgradually decreased with the increase of latitude.(5)24populationswere obviously divided into two groups by UPGMA,through the Mantelcorrelation test,geographic distance and genetic distance werepositively correlated,but not significant(r=0.182.p>0.05).