Association Analysis Of Molecular Makers With Growth Traits In Swimming Crab (Portunus Trituberculatus)

In order to improve the work of artificial breeding and genetic breeding in Portunus trituberculatus, themicrosatellite DNA makers have been developed by public databases of expressed sequence tags (ESTs) inthe study. The work investigated the correlation between growth traits and these microsatellite makers or theAFLP makers. And the maker-assisted selection would be valuable to improve the production of P.trituberculatus.A total of1578P. trituberculatus EST sequences were downloaded from public databases and screened forSSRs using SSRHUNTER13.0software and150were selected for microsatellite makers in the study. Thesemicrosatellite makers were designed using the PRIMER5.0program. But only twenty-seven paris weredetected polymorphisms. For the27primer paris, the number of alleles at each locus ranged from3to14, withan average of6.8alleles per locus. The observed and expected heterozygosities ranged from0.233to1.000and0.160to0.789, respectively. While the ranges of polymorphism information content (PIC) were from0.222to0.875.There were ten paris of microsatellite makers and thirteen paris of AFLP makers were selected to analyze theassociation between these molecular makers and growth traits in an F1family which is obtained from Y1familyof P. trituberculatus. And there were85polymorphism locus were found at the AFLP makers. The SPSS19.0software was used to analyze the molecular makers associated with growth traits.The result shows that three microsatellite locus had significant differences with growth traits. PT101had asignificant associated with body weight, body height, full carapace width, carapace width, meropodit length ofthe claw, fixed finger length of the claw(P<0.05) and had a very significant associated with body length,meropodit length of the first peraeopod (P<0.01);PT39had a significant associated with body height(P<0.05);PT74a very significant associated with meropodit length of the first peraeopod (P<0.01).And resultsindicated that ten AFLP locis had significant differences with growth traits. A4f450had a significant associatedwith body weight, body height, body length, full carapace width, carapace width, meropodit length of the claw,fixed finger length of the claw(P<0.05); A4f178had a significant associated with body length, body height, fullcarapace width (P<0.05) and had a very significant associated with meropodit length of the first peraeopod(P<0.01); D4f140had a significant associated with carapace width, body weight, body height, fixed fingerlength of the claw, full carapace width, meropodit length of the claw (P<0.05); D3f190had a significantassociated with body weight, full carapace width, carapace width, meropodit length of the claw(P<0.05) andhad a very significant associated with fixed finger length of the claw (P<0.01); B4f210had a significantassociated with body height (P<0.05), and had a very significant associated with body length (P<0.01); ButB4f380、B4f310、B4f170、A5f420only had a significant associated with body weight(P<0.05); D1f200onlyhad a significant associated with meropodit length of the first peraeopod (P<0.05).