Effect Of Infection In Limited Feeding Of BALB/c Mice Challenged By Aerosolized Brucella

When the blight struck, nutritional factors are often been overlooked, actually, it plays animportant role in resistance pathogen attack and occupies a very important position.Malnutritioncauses the immune system functional disorder, so that the body vulnerable to bacterial, virusesand other microorganisms infection.healthy body’s immune response is fast and accurate to diseases, and when the body has been plunged into chronic malnutrition or disease, its defense systemwill become vulnerable and opportunities offered to diseases.Brucellosis is an important zoonotic diseases, caused huge economic losses and seriouspublic health problem in many countries around the world, it has extremely far-reaching impacton the livestock industry and difficult to completely eliminate.Office International desEpizooties (OIE) have classified brucellosis as a prevalence of a variety of animals, DiseasePrevention Act have listedbrucellosis asB infectious diseases,including SARS, AIDS, and otherhighly pathogenic avian influenza.The disease can be infected by contact with skin and mucousmembranes, can be transmitted through the digestive tract, but also pollute the environment inaerosolsform,Lead to respiratory infections.In this study, Balb/c mice used to establish different levels of dietary nutrient conditions(normal feeding group and limited feeding group) Brucella infection in a mouse model byaerosol mode. Evaluate its pathogenic immune response and are triggered by differences in lungtissue proteomics technology to aerosol particles leads to the expression of the protein wasanalyzed to build spectrum,obtain critical data of regulatory proteins in the invasion process oforganism against bacterial aerosol nanoparticles, in oder to provide a theoretical basis for a clearaerosol Brucella infection pathogenesis and immune protective effect of the organism, Whileobserving the different trophic levels in mice infected with Brucella pathogenic effects anddifferences in the immuneresponse.The study exposure Brucella in the shape of aerosol using TSI3079Aerosol Generator andtest the diameter and density of aerosol particle by APS3321aerodynamic particle sizerspectrometer. According to preliminary experiment,the low and high infection dose of animalmodel is109and106respectively. During acute infection, mental state was undifferentiatedbetween mice in aerosol group and control group, while after exposure1d,3d,7d and15d,thelungs of mice were swelling and hemorrhage, spleen had been larger significantly. Grinding the lung and spleen infected for bacterial culture and PCR approval test, Brucella had been isolatedfrom the lung and spleen, it is time-and dose-dependent. This proves that Brucella can infectmice through aerosol, and colonize in tissues such as lung and spleen. The transmissionexperiments shows that mice can infect Brucella aerosol through contect.Pathological observation showed normal feeding mice after aerosol exposure1d,3d,7d,15d and its tracheal tissue morphology appear normal. However, restrictions on feeding group ofmice after infection7d,15d tracheal mucosal epithelial cell shedding. Lung pathology isobverise after infection.1d after the normal feeding mice were infected, the lung tissueappears different degrees of structural damage of the alveolar wall thickening. In restrictedfeeding mice after infected, which is mainly composed of lymphocytes and macrophagesinfiltration of inflammatory cells, capillary expansion congestion phenomenon, longer durationof infection,7,15days after infected, stove shape necrosis occurs gradually, serious damage inleft pulmonary than in the right. Cytokine detection of infection in different time changes byfluorescence quantitative PCR, we found that in normal diet group which were infected,Th1-type cytokines(IL-2、1L-12、IFN-2、TNF-2) RQ values were25.4、21.02、40.33、27.18on the third day, significant difference(p<0.01); Restricted diet group RQ values were72.45、366.5、54、75.6, significant difference(p<0.01). Both sets of Th2-type cytokines (IL-4, IL-10)expression levels increased first and then decline, Normal feeding group and restrict feedinggroup IL-4in third day and RQ peak81.45,153.91, compared to the uninfected group,significant difference((p<0.01); IL-10expression in both groups, there is a big difference,restricted feeding group RQ value reached37.45on the third day, the difference was significant(p <0.01); The highest expression of the normal diet group appeared on the first day RQ value isonly1.66, difference was not significant (p>0.05). Flow cytometry detect CD3, CD4, CD8,CD19antigen expression level. We found that Brucella aerosol infection can cause CD3+Tcells and CD4+T cells increase in normal feeding group, CD8+T cells and CD19+T cellswere not significantly different. In the restricted feeding group, CD3+T cells and CD4+T cellsincrease significantly, and CD19+T cells decrease significantly. Seen from the specific results,Brucella infected organism, cell-mediated immunity is a key Immunization form, since humoralimmunity is an auxiliary form, which also confirms the Brucella intracellular parasites propertywhile mice more susceptible to lack of nutritionIn order to study the regulation of lung tissue protein expression by Brucella aerosolchallenged of mice.using2-DE electrophoresis and mass spectrometry, we screened24proteinspots and identified16kinds of protein while the function involves cytoskeleton, migrationmovement, redox, inflammatory response, the response pressure, anti-cancer effects and so on. These results provide a theoretical basis for further research on Brucella pathogenic mechanismfor the development of drugs and the new targets and opportunities of treatment on respiratorysystem.