The Influence Of Trichinella Spiralis And Excretion/Secretion Antigens On Differentiation, Development And Function Of Dendritic Cells In BABL/c Mice

Trichinella spiralis is a kind of endangering serious foodborne zoonotic parasites which canform a long-term infection in skeletal muscle of more than150kinds of mammals, birds, reptilesand human. Dendritic cell is the most powerful antigen presenting cell in the body, it not onlycan also start the primary immune response in the first time and activate na ve T cells strongly,but also determines the direction of the body’s immune response. Many studies have alreadyconfirmed that there are some subsets of dendritic cell inhibited the body’s immune response inthe process of differentiation and development, many parasites can evade the host immuneresponse, causing along-term stability infections in many parasitic infection. To analyze theimpact of Trichinella spiralis in DC differentiation, we use Trichinella spiralis susceptibleBALB/c mice to establish infection model and PBS control group. In the process of Trichinellaspiralis chronic infections, application methods of flow cytometry was used to detect somesubsets (CD11c+CD11b+CD8a+, CD11c+CD11b+CD8a-, CD11c+B220+CD8a+,CD11c+B220+CD8a-) and surface molecules(MHC-II, CD40, CD80, CD86) of DC afterinfected14,21,28,35,42days. The results showed that when the BALB/c mice infected withTrichinella spiralis, the number of DC subsets are varying degrees of increase in the differentperiods, and DC surface molecules expression increased. These results indicate that the numberof DC subsets have increased in the different period after infected with trichinella, it shows thatthe Trichinella spiralis chronic infection induced DC in different subsets proliferation anddifferentiation that in spleen and MLN, at the same time, the increasing expression of surfacemolecules shows that Trichinella spiralis and excretory/secretory antigens can stimulate theactivation of DC in mice to make the immature DC maturation, but the level of maturity isdifferent. This initially revealed the role of DC in the chronic infection with Trichinella spiralis.In addtion，Trichinella spiralis may modulated the host immune balance and reduced resistanceby affecting the functions of DC, so they can continue to multiply and grow in the host body.