Pathogenicity Comparison Between H5N1and H5N5Subtype Avian Influenza Virus Isolated From Duck To Mice

Avian influenza（avian influenza，AI） is poultry viral infectious diseases causedby the avian influenza virus of influenza virus type A, the main cause systemic orpoultry respiratory infectious diseases.China’s agriculture ministry has been classifiedas A class of highly pathogenic avian influenza epidemic. Internationally is also listedit as one of the anti-terrorist bio-content. Through the avian influenza virus etiologyand epidemiology survey, especially waterfowl duck is a huge repository of avianinfluenza virus, and our waterfowl habitat accounts for about70%of the worldwide.Since our country duck farms are distributed in lakes and rivers, and migration occursduring the breeding season. This makes it easily accessible to domestic waterfowl andwild waterfowl such as chicken, pigs and other domesticated livestock, makingwaterfowl become an important intermediate host, causing the spread of avian flu.Therefore, the study of pathogenic avian influenza virus in mammals, the research inthe field of influenza virus is particularly necessary.In this study， H5N1subtype of avian influenza virus from ducks（A/Duck/Liaoning/N/2011）and H5N1subtype of avian influenza virus from ducks（A/Duck/Changchun/01/2010）diluted to a concentration of virus solution virus10-7EID50/ml, inoculated with0.2ml attack agents were to BALB/C mice. After miceinfected with the two viruses, the mice were not any significant clinical symptoms,body weight, appetite, and mental status were unaffected. The third, fifth, tenth,fourteenth days after infection, the mice were taken lung, brain and liver virusinoculated into chicken embryos to do regression experiments. Take the death of chickembryo allantoises liquid for HA, HI and RT-PCR identified, but only after the fifthday of infection were detected in the lungs and liver of H5N1and H5N5subtypes ofthe avian influenza virus, indicating that mice resistant to over-after the transfer ofinfluenza virus showed positive trends. Meanwhile feces of mice by RT-PCR andfound no signs of detoxification. After the samples were collected and H5N5subtypeH5N1subtype avian influenza virus infected mice on day5of the brain, lungs, liver,spleen and kidneys, found by histopathology, virus present in the organs of mice andcells in mice organs and tissues produce different pathological damage to the lungs and liver is most obvious. But pathological damage caused by the H5N1strain ofmouse lung tissue severe than H5N5subtype, causing damage to the liver pathologysubtype H5N5was not serious. The results of this study was to investigate the subtypeH5N1and H5N5subtype avian influenza virus pathogenicity in mice were induced,and pathogenic differences between the two viruses. For the avian influenza virus inmammals provides a theoretical basis for pathogenicity studies for further study ofH5N1and H5N5subtype avian influenza virus pathogenesis in mammals provides acertain reference value.