| In order to alleviate the current situation of Taxus chinensis germplasm resource scarcity and improve thequality of artificial planting Taxus chinensis, we used the gibberellic acid(GA3) to treat the biennial yew Taxuschinensis to strike water root.Hydroponic and soil cultured Taxus chinensis’s organization form, physiology andbiochemistry and molecular level were compared to explore the influence form water to Taxus chinensis’smorphological structure, physiology and biochemistry and related gene expression, thus revealing the rules fromsoil culture to hydroponic of Taxus chinensis, providing scientific theory basis for large fileds of soillesscultivation of Taxus chinensis.The results showed that the optimal inducing concentration of GA3promoting yew rooting of water root was400mg/L. The cells in hydroponic yew root structure were more compact than those in soil. Developedaerenchyma and lack of vascular cylinder cell and root hair were observed in hydroponic group by paraffinsection.The contents of pigment in hydroponic group were lower than that in soil cultured group, although the rootactivity was higher than soil cultured group(p<0.05) and the activity of SOD, CAT and POD in hydroponic groupwas higher than that in soil cultured group (p<0.01), while the MDA content, root damage rate of conductivity androot total protein showed no significant difference than that in soil cultured group. Fluorescence quantitative PCRresults showed that lipoxygenase2(lox2) expression quantity was higher than that soil cultured group(p<0.05),while cytochrome c reductase450(p450)expression quantity was less than that soil cultured group, into extremelysignificant difference(p<0.01). Our studies suggested that hydroponic could change the root structure, the pigmentcontent, part corresponding physiology and biochemistry and related gene expression of Taxus chinensis to adaptto low oxygen environment during the hydroponic process. |
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