Identification Of Adhesion Protein In Mycoplasma Capricolum Subsp. Capripneumoniae

Mycoplasma capricolum subsp. Capripneumoniae belongs to the Mollicutes, is a memberof the Mycoplasma mycoides cluster, can cause the Contagious caprine pleuropneumonia ofgoatsThe adhesion of pathogens to host is the first step to mycoplasma infection, is oneimportant aspect of pathogenesis research. Although some mycoplasma adhesion proteins havebeen identified, but mainly concentrated in Mycoplasma Genitalium，Mycoplasma pneumoniae，Mycoplasma bovis，mycoplasma agalactiae，Mycoplasma suis. In the word, Mccp adhesionproteins are not reported. Further study of the adhesion process of Mccp is very important forprevention and control of Mccp infection. On the basis of whole genome sequencing of Mccp87001, according to bioinformatics, select a717bp P0297genes, focuses on its adhesiveproperties may have.P0297gene of Mccp87001encodes a27ku prote P0297. The P0297gene was cloned andexpressed in E. coli to obtain recombinant P0297(rP0297). Using a BALB/c mice antibodyagainst rP0297demonstrated that P0297is a membrane protein of Mccp with western blotanalysis. Goat tracheal epithelial cells processed by paraformaldehyde is incubated with rP0297and Mccp respectively. Immunostaining visualized via confocal laser scanning microscopyshowed that rP0297and Mccp were able to adhere to fixed goat tracheal epithelial cells, and thiswas also confirmed by a sandwich ELISA, devised to investigate interaction betweenrP0297/Mccp and membrane and cytoplasmic protein fractions of goat tracheal epithelial cells.All the adhesion phenomenon can be inhibited by antibodies. The experiment confirmed thateventually P0297is the surface lipoprotein of Mccp. P0297has obvious adhesion effect to thegoat tracheal epithelial cells.