The Apparent Digestibility Of Eighteen Feed Ingredients For Jian Carp (Cpyrinus Carpio Var.Jian)

This study was fouced on the apparent digestibility(AD) of dry matter(DM), crude protien(CP), ether extract(EE), essential amino acid(EAA), total phosphorus(TP) and gross energy (GE) of six animal protein feed ingredients, seven plant protein feed ingredients and five energy feed ingredients for Jian carp (Cpyrinus carpio var.Jian). It would provide theoretical basis for optimizing the feed formular of Jian carp. And then, the study was determined the apparent digestibility of different nutrients of nine feed ingredients for Jian carp through two different fecal collection methods, which was fouced on compareing the difference bewteen two fecal collection methods for Jian carp.Chapter 1:Apparent digestibility of nine conventional feed ingredients for Jian carp (Cyprinus carpio var. Jian)The first experiment was conducted to study the apparent digestibility of DM, CP, EE, EAA, TP and GE of fish meal, meat and bone meal, soybean meal, peanut meal, rapeseed meal, cottonseed meal, DDGS, wheat middling and rice bran for Jian carp (Cyprinus carpio var. Jian). The apparent digestibility was determined using a reference diet with 0.5% chromic oxide as indicator and test diets that contained 70% reference diet, by weight, and 30% of the test ingredients being evaluated. Jian carps (average weight:200.0±4.7 g) were stocked in rectangular fiberglass tanks at a density of 10 fishes per tank. Feces were collected from triplicate groups of fish by net fishing. The apparent digestibility of dry matter ranged from 35.44% to 71.10% and the values of peanut meal and soybean meal (71.10% and 65.17%) were higher than other test ingredients. The apparent digestibility of crude protein and phosphorus for the test ingredients ranged from 60.31% to 88.75% and 6.52% to 58.21%, respectfully.Chapter 2:Apparent digestibility of nine unconventional feed ingredients for Jian carp (Cyprinus carpio var. Jian) The second experiment was determined the apparent digestibility of DM, CP, EE, EAA, TP and GE of blood meal, silkworm pupa meal, expanded feather meal, hydrolyed feather meal, protein peptide, corn gluten meal, corn, broken rice and barley grain for Jian carp (Cyprinus carpio var. Jian). The test diets were consisted by 70% reference diet and 30% test ingredients containing 0.5% chromic oxide as an inter marker. Three hundreds fishes with the average weight of (220.53±4.67 g) were reared in door aquarium tanks (3.0 m×0.8 m×0.8 m), and after feeding the test feed for a week, the fecal samples were collected by net fishing. The results showed that the apparent digestibility of DM, CP, EE, EAA, TP and GE were ranged from 63.11% to 94.57%,66.51% to 92.85%,69.73% to 93.20%,66.48% to 88.32%,29.04% to 98.92% and 58.67% to 91.92%, respectively. And the apparent digestibility of nutrients for corn gluten meal were the highest value than other test ingredients (P<0.05). The apparent digestibility of CP for expanded feather meal was the lowest and the values of other ingredients were more than 75%. The apparent digestibility of TP for protein peptide was 54.13% and the values of blood meal, silkworm pupa meal and expanded feather meal were above 40%, while the value of hydrolyed feather meal was the lowest (29.04%).Chapter 3:Reserch of apparent digestibility of feed ingredients for Jian carp (Cyprinus carpio var. Jian) by two methods of fecal collectionThe third experiment was determined to evaluate the apparent digestibility of of DM, CP, EAA and TP of blood meal, silkworm pupa meal, expanded feather meal, hydrolyed feather meal, protein peptide, corn gluten meal, corn, broken rice and barley grain for Jian carp (Cyprinus carpio var. Jian) in two different fecal collection method (siphoning method and dissection method). The apparent digestibility was determined using a reference diet with 0.5% chromic oxide as indicator and test diets that contained 70% reference diet, by weight, and 30% of the test ingredients being evaluated. Three hundreds fishes (average weight:220.53±4.67 g) were stocked in rectangular fiberglass tanks (3.0 m×0.8m×0.8 m) at a density of 10 fishes per tank. After feeding the test feed for a week, the fecal samples were collected in siphoning method and then in dissection method at the end of culture experiment. The result showed that the apparent digestibility of DM, CP, AA and TP of the test ingredients in siphoning method were higher. The apparent digestibility of CP in two different fecal collection methods were significantly different, and the value of corn gluten meal in two fecal collection methods were both in high levles. The apparent digestibility of essential amino acids were higher than that of crude protein in two fecal collection methods, and the differences were found among digestibility of individual amino acids.In conclusion, the digestibility information could prove that peanut meal, soybean meal and corn gluten meal are good plant protein sources for Jian carp, and they can be used to instead of part of fishmeal in actual feed production. And wheat middling and corn are good energy sources for Jian carp. While because of the different produce process of animal protein ingredients like feather meal, the nutrients compositions are different between the products, and that could lead to the different apparent digestibility. So the nutrients compositions of these feed ingredients must be tested before formulating the feed. By comparig between two fecal collection methods, the apparent digestibility in siphoning method could be more accurate and more scientific.