The Ultrastructure Of Male Accessory Gland Development From Gampsocleis Gratiosa

The structure of male accessory glands of Tettigoniidae is complex. The main functionsof secretions of male accessory glands are formation of spermatophore at mating that consistsof sperm, enhanced oviposition and reduced mating receptivity of female. Male accessoryglands of Gampsocleis gratiosa Brunner von Wattenwyl consist of two bilateral masses,locate on inner side of testicles and on ventral surface alimentary canal, each of which iscomposed of numerous tubules. According to external form, they are divided into three types:long opalescent gland, short opalescent gland and hyaline gland. Three types of glands andspermaduct open into the ejaculatory duct, which locate in the end of abdomen.We examined the change of different developmental periods of male accessory glandsfrom Gampsocleis gratiosa using transmission electron microscope. The results indicated thatlong opalescent gland and short opalescent gland are fully differentiated in last-instar nymphbut hyaline gland after eclosion. In epithelial cells, organelles undergo the change fromdispersed to concentrative and few to more during male accessory glands development.Secretions undergo the change from few to more and are filled in whole gland lumen inmature period. All gland tubules of male accessory glands are histologically similar, whichconsist of peritoneal membrane, muscle layer, basal lamina and single epithelium. Theepithelium lies on a thin basal lamina surrounded on the outside by a tunica of striated musclefibers and connective tissue rich in tracheole. From last-instar nymph to eclosion (tenth day),the epithelial cells of gland tubules are not single, while after eclosion (ten days later), aresingle. In mature period, there are abundant rough endoplasmic reticulum, mitochondria andGolgi apparatus in the epithelial cells, displaying features of protein synthesis and secretion.There are four types of secretions in gland lumen, including electron-transparent material,electron-dense granules, Fibrillar material and villous spherical material.The embryonic development of Gampsocleis gratiosa is examined by stereo microscope.The results indicated that embryonic development of Gampsocleis gratiosa are grouped into6phases: formation and differentiation of embryonic primordium, anatreps, katatrepsis, dorsalclosure, completion and hatching.