Research Of AM Fungal Distribution And The Relationship With Soil Carbon And Nitrogen In The Rhizosphere Of Lonicera Japonica

Lonicera japonica is a perennial semi evergreen vine from Caprifoliaceae family; it is a kind of important herb plants. Arbuscular mycorrhizal fungi distributed widely in nature and can establish symbiotic relationships with the roots of plants, it can promote the roots of host plant to absorb water and nutrients to enhance the resistance, regulate the secondary metabolism action, promote growth, increase yield and improve quality of host plant. As an important component of the ecological system, AM fungi is changing the micro-environment of the roots. This paper focused on the relationship between AM fungi and Lonicera japonica. We collected the root samples and soil samples in a full year, and then we studied the ecological distribution of AM fungi and change of soil factors. Following is the main results:1. AM fungi can form a good symbiotic relationship with Lonicera japonica. It formed Arum-type mycorrhizas. After continuous evolution, the morphological structure of AM fungi has been produced in the adaptability.2.15AM fungal species of4genera were found in the rhizosphere of Lonicera japonica. Of them, there were nine to the genus Glomus, five to Acaulospora and one to Scutellospore. Glomus is the dominant genera, the next in order is Acaulospora and the Scutellospore is lowest. Different months had different importance value and dominant species. The importance value of G. multicaule, G. reticulatum, A. bireticulata are the highest in every months, it indicate they are dominant species in the rhizosphere of Lonicera japonica.3. AM fungi in the rhizosphere of Lonicera japonica have a significant temporal and spatial distribution. The maximal value of spore density was observed at the0-10cm layer, and then gradually decreased with soil depth. In different months, spore density was significantly changed. The highest vesicular colonization appeared in December of2010, and the lowest vesicular colonization appeared in June of2011. Arbuscular colonization is always low and the highest value appeared in April of2011. Soil factors and the season affect AM fungal colonization.4. Glomalin is an important source of soil organic C and total N. Total N was very positively correlated with spore density, external hyphal colonization, easy extracted glomalin and total glomalin. It was positively correlated with internal and total colonization. It was positively correlated with vesicular colonization. Organic C was very positively correlated with spore density, easy extracted glomalin. It was positively correlated with total colonization. Stepwise regression analysis shows that, the content of EEG and TG are influenced by spore density.The results showed that Lonicera japonica establish well symbiosis with AM fungi. There have obvious spatial-temporal heterogeneity in ecological distribution and species diversity and affected by soil factors and season. Glomalin is an important source of soil organic C and total N, and soil quality was improved by the cumulative effect of glomalin. Using mycorrhizal technology in the plant cultivation of Lonicera japonica, it not only can increase the production of the plant, but also can improve the quality of the soil and promote the soil ecological system benign circulation.