The Preliminary Study On Artificial Propagation And Biochemical Component Transfer Of Japanese Eel

Japanese eel is a kind of high economic value fish and cultivate in the worldwide.It is with rapid growth, fleshy delicate, rich nutrition, taste delicious, etc. Since 1980s breeding japanese eel is developing very fast , China has become the world’s largest breeding japanese eel country. However, because of all japanese eel needed to fry breeding rely on natural fishing, The fishing methods led to nature eel seedings resources serious recession. So in japanese eel artificial breeding related research seems more and more necessary .This paper adopts carp pituitary and HCG ripened japanese eel, get the mature sperm and eggs. Adopt 17 alpha hydroxy progesterone and carp pituitary gland oxytocin female japanese eel spawning rate achieved 80%. Carp pituitary and HCG can also prompted females ovulation but spawning rate less than 10%. Uefa ketone and salmon to release hormone analogue cannot prompted females ovulation .Using different hormone injections tofemale eel, determination analysis different tissue protein, fat, moisture, ash , calcium and phosphorus content. The results found that after 10 times injected carp pituitary gland and HCG the calcium quality score (mg %) in bone down significantly respectively achieved (746.3±90.4) percent and (747.7±116.6) percent. Compared with the control and blank group significant difference (p﹤0.05), quality score respectively for (1020 + 21.7) percent and (1015.7 + 25.5) percent. Serum calcium content decline first, on the 14th day achieve minimum (0.55±0.17)mol/l and (0.534±0.11)mol/l then rise on the 70th day achieve maximum (1.193±0.2)mol/l and (1.187±0.22)mol/l. The calcium content in gonadal and liver has significantly increased , the control and blank group have little change , compared with the experimental group have significant difference (p﹤0.05).Experimental group phosphorus in bone decline , on the 70th day achieve minimum (0.97±0.4)percent and (0.99±0.47) percent, compared with the control and blank group there were significant differences(p﹤0.05). Serum phosphorus first decline,on the 28th achieve minimum (1.494±0.85)mol/l and (1.168±0.73)mol/l,then begin rise. Phosphorus in ovarian increase on the 70th day reach maximum (213.3±73.3)mg/100g and (212.2±83.7)mg/100g, compared with the control and blank group have significant difference(p﹤0.05). The phosphorus in liver have no significant change .Protein content in liver and gonads of four group decreased , the control and blank group declines much more significant , compared with the experimental group have significant difference (p﹤0.05). Protein content in muscles and bones have no significant change. Fat content in muscle of four groups decreased, The experimental group decline more significant, respectively achieved (43.8±1.92) percent and (43.5±0.97) percent, compared with control and blank group have significant difference (p﹤0.05). The fat in liver and gonads rise significantly, compared with the control and blank group there were significant differences (p﹤0.05). The fat in bone have no significant change.Except for the water in liver decline other organization all rise, compared with the control and blank group have significant difference (p﹤0.05). The ash content in the muscle and liver dropped significantly, compared with the control and blank group have significant difference (p﹤0.05). Ash in bones and gonads have no significant change.During the experiment organizations(except the serum phosphorus) between the control and blank group have no significant differences(p﹥0.05), In the 70th day between the two experimental group have no significant differences.Experimental results showed that injection hormones prompted female Japanese eel ovarian growth used the body storage nutrition. The calcium in bone is used, it through the blood circulation transferred to ovarian and liver. Phosphorus also through the blood circulation transfer to the ovarian. Meanwhile the liver synthesize protein increased , fat in muscle declined significantly . At this time the protein and fat content in ovary compared with the control and blank group significantly increased (p﹤0.05). These changes show that the female eel ovarian growth mobilized body storage nutrients , provide material base for breeding offspring.