Study On Genetic Structure Of Wild Mice In China

GWAS has in a very important position in mouse genetic study at this period. But there is many blockade in the process because of non-abundant genome polymorphism, hence, the researchers need to find another more rich resource. Wild mice have more complicated environment, longer evolution history, low linkage disequilibrium, and high SNP density,which become a excellent resource to study GWAS. Our country has a wide geography and abundant resource to discover,so this study will focus on genetic polymorphisms of wild mice subspecies in China.The sampling regions of this study covered 13 provinces and 36 localities, which are related to 7 zoological regions in China. The study was performed to investigate the genetic polymorphisms of wild mice following major approaches:(1) Using LDR genotype to analyse mitochondrial control region SNPs background.(2) Using F-CSGE and DNA sequencing to discover and genotype SNPs.(3) Base on SNP data on Chr 1,we conduct LDR to analyse SNPs and testify subspecies assortment.Results:(1) We find 9 haplotypes in mouse mitochondrial control region,Hl,H5 and H9 are major haplotypes, and their rates are 37%,16% and 29.6%.The others are related to the majors. According to the sequences blast, we find H1 is same with M.m.castaneus,and H5,H9 are same with M.m.musculus. Those haplotypes divide our wild mice into south-north subspecies.(2) Y haplotypes have two majors,Hl and H2,their rates are 20.31% and 40.63%.The wild mice in Henan, Heilongjiang, Shandong, Liaoning, Xinjiang belong to H1 haplotype. Wild mice in Jiangsu, Zhejiang, Shanghai, Yunnan and Hubei belong to H2 haplotype. (3) We pick 9 SNPs as Tag-SNPs to construct 9 haplotypes. A1 and A2 are major haplotypes, their rates are 50.67% and 25.33%.They distribute to the both sides of Yangtze.The result is accord with the MIT coding regions.The extensive genetic polymorphisms and great impact of human activities upon the distribution of the studied species were confirmed by this project. M.m.castaneus and M.m.musculus geographic structures in China were specified. The study implies that the taxa found in China can be used for genetic dissection of complex traits as these resources are highly complementary to established laboratory stocks.