The Structure Of Anammox Community And Its Relationship With Environmental Factors In Sediment Of Snakehead Aquaculture Enclosure

Nitrogen pollution in high-density intensive freshwater aquaculture mode has becme one of the bottlenecks in the healthy development of freshwater aquaculture.The current freshwater aquaculture need to find a rapid effective method to solve the nitrogen pollution of aquaculture water urgently. Anammox bacteria that discovered in recent years has denitrification capability. It is not necessary to add external carbon source, and the reaction product has no secondary pollution, its denitrification is an effective way to solve nitrogen pollution of aquaculture systems.Firstly, the experiment used PCR-DGGE technique to investigated microbial community structure in sediment of Snakehead Aquaculture Enclosures located in Guangdong Nansha District. In order to understood whether Anammox bacteria existed in sediments of freshawater pond, and the microbial communities in its living environment. Then used high throughput sequencing technology to get information of Anammox community structure in sediments of Snakehead Aquaculture Enclosures,and analysised its relationship with environmental factors, to get key environment factors that affecting Anammox community structure in aquaculture systems.Provided basic data for the subsequent aquaculture system useed Anammox bacteria to reduce the nitrogen pollution.The experiment used PCR-DGGE to analysis the microbial communities in sediment of Snakehead aquaculture enclosure. We found that Anammox bacteria was existed in sendiment of Snakehead aquaculture enclosure, accounting for 2% of the cloned sequences.The structure of microbial community in sendiment of Snakehead aquaculture enclosure, it was mainly composed of Proteobacteria, Actinobacteria,Chloroflexi, Bacteroidetes, Acidobacteria, Nitrospirae, Planctomycetes, Firmicutes,Gemmatimonadetes. The structure of microbial community changed nonsignificant(P>0.05).Experiment used high-throughput sequencing technology to analysis the Anammox in sediment of Snakehead Aquaculture Enclosure. The community of Anammox bacteria was composed by Candidatus Brocadia and Candidatus Anammoxoglobus, the bacteria of the genus Candidatus Brocadia accounted for99.6% of Anammox total community OTU’S. The genera of Candidatus Brocadia bacteria normally presented in the nitrogen pollution of the environment. Inferred nitrogen pollution of Snakehead Aquaculture was serious.The Anammox bacteria communities was difference significantly in aquaculture process(Rho=0.599,P<0.01). CLUSTER and MDS cluster analysis concluded that Anammox community structure can be divided into prebreeding, breeding and post-breeding three community according to month. Environmental factors NO3-、TFe and NH4+ were the optimum combination of environment factors for Anammox bacteria community(Rho=0.79, P<0.01). The possible reason was that these environment factors were the Anammox reaction substrates, etc.This study showed that Anammox bacteria was existed in sediment of Snakehead Aquaculture Enclosure. The community of Anammox bacteria was composed by Candidatus Brocadia and Candidatus Anammoxoglobus, Candidatus Brocadia was the main part of the entire community. The bacteria of the genus Candidatus Brocadia accounted for 99.6% of Anammox total community OUT’S. Anammox bacteria community was evolving constantly in the aquaculture process(Rho=0.599,P<0.01).NO3-、TFe and NH4+ were the environment factors that affected the structure of Anammox bacteria community in environment factors(NO3-、TFe、TMn、NH4+、SO42-、TOC and TN)of sediment pore water(Rho=0.79, P<0.01). This study proved the distribution and the structure of Anammox community in freshwater aquaculture.Provided a basic data for the subsequent aquaculture system use Anammox bacteria to reduce the nitrogen pollution.