Effects Of Efficient Cellulose Decomposing Bacteria On The Capabilities Of Degradation And Deodorization To Pig And Cattle Manure

With the development of modern animal husbandry, farming has been transformed from thousands of households farming model to large-scale and intensive farming, but also comes with a lot of focus on pig and cattle manure. If large amounts of manure were not timely and effective solved. It will cause a lot of environmental pollution and affecting food safety and human health. How to take advantage of these abundant biomass resources, it has become the current issues for economic and environmental that are need to be solved. In this study, pig and cattle manure adding different proportions of sawdust(0, 5%, 10%, 15%) as a matrix, un-supplementation or supplementation(5%) efficient cellulose microbial community in the matrix.Determination of the moisture content and p H value in the process of fecal degradation and using DNS method, potassium dichromate method, Kjeldahl method,acid method, boric acid absorption, Zinc ammonium salt absorption colorimetric method determined carboxymethyl cellulose enzyme activity(CMC enzyme activity),total organic carbon(TOC), total nitrogen(TN) content, crude fiber content, NH3 and H2 S Carbon dioxide emission. Through those index, we study the effect the efficient cellulose decomposing bacteria on degradation pig and cattle manure. The results are as follows:1、Compared with non added cellulolytic strains, adding cellulose microbial community increased the moisture content degradation, and the effect of degradation significantly(P<0.05) decreased in pig manure. The p H value was 7.80-8.34 throughout the experimental period when supplementation cellulose microbial community and adding sawdust, the p H value decreased on the 20 days.2、In the adding cellulose microbial community treatment group but did not add the sawdust, no significant differences CMC enzyme activity were found in the process of cattle manure degradation, However, the CMC enzyme activity was significantly(P<0.05) higher than that of without adding cellulose-decomposing microbes group, even the highest enzyme activity reached 2.45(mg·m L-1) on the 10 d,15d and 25 d in the process of pig manure degradation. Adding 10% sawdust, theCMC enzyme activity obviously higher than that of 5% and 10% in the group of pig manure. Without added cellulose microbial community and adding sawdust reduced the CMC enzyme activity.3、The cellulose microbial community decreased the TOC content, and increased the TN content in all the treatments. After adding 15% sawdust increased32.34% and 33.95% the content of TN, TOC decreased 29.48% and 32.94%respectively in the pig and cattle manure.4、The cellulose microbial community reduced the crude fiber and the degradation rate were 36.64%, 43.03% in the pig and cattle manure respectively, and significantly(P<0.05) higher than that without bacteria groups.5、The bacteria can reduced the NH3 and H2 S release, and the reduction rate of NH3 and H2 S release were 82.7%, 62% and 60.4%, 70% of the total reduction rate of the test in pig and cattle manure in the first of 15 days. Adding 10% and 15% sawdust significantly(P<0.05) inhibited the release of NH3, and 10% sawdust significantly(P<0.05) inhibited the release of H2 Sin the process of degradation in the cow manure.The results showed that cellulose microbial community has some dehydration effect and it can control p H value, can improve the activity of CMC, degrade the fecal crude fiber and organic matter, inhibit the release of H2 S and NH3 in the process of degradation of pig and cattle manure. Adding 10% sawdust promoted the cellulose microbial community of pig and cattle manure degradation ability and play obviously deodorizes and increase the nitrogen content role. Under the local climate conditions,the result could provide reference for the treatment of pig and cattle manure resources and reduction.