Synthesis Of Copper Chalcogenides Micromaterials And Their Applications In Biochemical Analysis

With unique optical, electrical, thermal, magnetic properties, transition metal-VIA compounds have become one of the research hotspots in fields of chemistry, physics and materials etc. Copper chalcogenides nano/micro materials have been widely concerned because of their excellent properties, such as catalysis, luminescence, gas sensing, photothermal and photoelectric conversion etc. At present, the research of copper chalcogenides nano/micro materials is still in the primary stage mainly focused on their synthesis and properties, which belong to the basic research category. Moreover, the performances and applications of nano/micro materials depend on their morphologies, sizes, compositions, structures and synthesis methods etc. Thus, it has practical significance to explore the new synthesis methods to prepare the copper chalcogenides nano/micro materials with different compositions and morphologies, and investigate their properties and applications. In this study, the hollow porous CuO microspheres and the copper selenides with different morphologies, phases and structural forms were prepared via a simple and green hydrothermal method. The catalytic property and intrinsic enzyme mimics activity of as-synthesized materials were also studied, suggesting they could be applied in the field of biochemical analysis. The main research contents were listed as follows:（1） A simple, green and template-free hydrothermal method was developed to prepare the fernwort-like 3D superstructures of copper selenide（CuSe） microcrystals in large-scale by using polyvinylpyrrolidone（PVP） as both reductant and surfactant. Time-dependent morphologic evolution was studied in order to explore the formation mechanism of the as-prepared products. The copper selenides with different morphologies, phases and structures could be prepared by varying the synthesis parameters, such as precursor molar ratio, precursor combination and the molecular weight of PVP. The fernwort-like 3D superstructures of CuSe microcrystals show excellent fenton-like catalytic activities in the degrading of malachite green（MG） and rhodamine B（RhB）, thus it could be used to purify organic dyes wastewater.（2） The hollow porous CuO microspheres（CuO MPs） were prepared via a simple and efficient hydrothermal method with NaHCO3 providing CO2 microbubbles to act as “soft template”. The morphologic evolution process of hollow porous CuO MPs at different concentrations of NaHCO3 was explored. Moreover, the hollow structure with a porous shell of CuO MPs might be more attractive than solid or closed hollow structures in some aspects, such as the high surface area, high absorption capacity and good reaction activity. Therefore, we developed a novel and simple fluorescence sensor for glutathione（GSH） based on hollow porous CuO MPs-O2-TPA system, with a detection limit of 0.2 μmol/L in the linear range from 0.5 to 100 μmol/L. And what’s more, it also could be applicable to the determination of GSH in serum samples.（3） A simple, novel and clean colorimetric sensor was developed for the detection of Hg²⁺ based on the intrinsic oxidase-like activity of hollow porous CuO MPs. It could catalyze the oxidation of 3,3’,5,5’-tetramethylbenzidine（TMB） into a blue colored cation radical without addition of H₂O₂ as an oxidant. Then, according to the colour（absorbance） change of CuO MPs/TMB system after adding GSH and Hg²⁺ successively, to realize a highly sensitive detection of Hg²⁺.In summary, we aim to develop a simple and green hydrothermal method with good applicability and popularity to synthesize copper chalcogenides with different morphologies, phases and structures, such as fernwort-like 3D superstructures of CuSe microcrystals and hollow porous CuO microspheres. The relationship between structures and properties of the as-prepared materials were further investigated. At last, we make a preliminary attempt to apply copper chalcogenides nano/micro materials to the field of biochemical analysis.