Detection Of Biomolecule By Nanochannel And Investigation On The Influence Factors

Nanochannel is the pores or tubules with diameter from 0.1 to 100 nanometers. Because of its structural stability, better thermal stability, excellent chemical and optical properties in the micro scale, nanopore has potential applications in the field of major scientific research. Especially the significance in the detection of biomolecules, the relevant research has been carried out at home and abroad. Many new works on analyzing and detecting the certain protein molecule by nanochannel are waiting us to do. Therefore, investigation the migration velocity and the flux of biomolecules in nanochannel by changing the temperature and viscosity of electrolyte solution. Hereby, main works in the present thesis are shown as follows:1. Design and assembly of the biomolecule detection device which can measure the ion current and record it. The experimental instruments and the platform were built up, realization of detecting objective particles by recording the changing of measured current. Study the influence of environmental temperature on IgG particles through nanochannel. Recording the ion current in different temperature which caused by IgG particles, we can find the inflection point of ion current changes with the temperature. To explain and verify the phenomena, a reasonable model was established and simulation of IgG particles with C++ program.2. Investigation the impact of solution’s viscosity and concentration gradient on migration of IgG and BSA particles. Viscosity can affect particles’motion by changing the drag force in solution. Increasing viscosity of solution can effectively decrease particle’ speed in solution, therefore reduceing the number of particles pass through nanopore in unit time. Because the shape and surface charge density of IgG and BSA are different, the effect of viscosity on particles is also different. Concentration gradient between nanopore can change the bidirectional electroosmotic flow in nanochannel and the potential near the nanopore, thus affecting the capture rate which particle are attracted by nanopore and migration time of paticles in nanochannel.3.A novel sensing devices are bulit on the basis of the chips containing nanopore arrays(PC) membranes and microproes in Si3N4 films, the transmembrane ion current induced by biomolecule’s translocation was recorded and analyzed. Detecting the current in multichannel and hybrid channel respectively. In hybrid channel, detailed translocation information is also extracted from discrete current blockages in basic current curves and the time of blocking signals. We can regulate the frequency of blocking signals by changing the number of effective nanopore in hybrid membrane. These results indicated that the nanofluidic device based on the chips integrated by micropores and nanopores possessed comparative potentials in biomolecule sensing.To sum up, the influence of experimental temperature, electrolyte solution and hybrid nanochannel on biomolecule through nanochannel were studied in this paper, which the foundation of detecting the specific biomolecule through nanochannel was established.