Synthesis And Electrochemical Application Of Semiconductor Chalcogenides

As a typical and important group of semiconductor materials, chalcogenide has aroused great concerns due to its special physical, chemical properties, optical and electrical properties. Thus, it has intriguing prospects in the application of optics, electromagnetism, biomedicine and electrochemistry, et al. In this article, three different kinds of chalcogenide were successfully synthesized by hydrothermal/sovelthermal method, which were utilized to immobilize enzyme/protein/antibody and fast, stable and simple biosensors. This work mainly included:1. The Sb2S3 material was synthesized through hydrothermal method, and its morphology, phase and elemental composition were characterized by Scanning Electron Microscopy(SEM), X-ray diffraction(XRD) and Energy Dispersive Spectrometer(EDS), respectively. Then, glucose oxidase(GOx) was immobilized onto the as-obtained Sb2S3 and IL films, gainning Sb2S3/IL/GOx-CPE modified electrode. Spectroscopic and SEM examination demonstrated that GOx retained its native activity on the Sb2S3/IL film. Electrochemical studies revealed that Sb2S3 material could adsorb GOx into its gap and facilitate the electron transfer between GOx and the work electrode. At the same time, the modified electrode revealed good electrocatalytic property toward glucose with wide linear range of 140μM-1000μM, low detection limit of 35.0μM.2. Using hydrothermal method, the rod-like Sb2Se3 was prepared and tested by SEM, XRD and EDS. On the basis, the Sb2Se3 material was dispersed into ionic liquid(IL) to form an uniform film for immobilizing GOx and Cyt c. The Sb2Se3/IL/GOx-CPE and Sb2Se3/IL/Cyt c-CPE were both characterized by UV-vis spectra(UV-vis) and SEM. Otherwise, electrochemical experiments indicated that the GOx and Cyt c were realized direct electron transfer(DET) based on Sb2S3/IL film. Furthermore, two kinds of biosenors were proposed and showed wide linear response to glucose of Sb2Se3/IL/GOx-CPE in the range of 8-1700 μM, nitrite(NO2-) of Sb2Se3/IL/Cyt c-CPE for 5-5300 μM, with a detection limit of 2.0 μM for glucose, nitrite(NO2-) for 1.2 μM, respectively. Therefore, the Sb2Se3 material is expected to construct multifunctional electrochemical biosensors.3.The sheet-like Bi2Se3 material was synthesized by solvothermal method and characterized structurally. Subsequently, the composite of Bi2Se3 and [BMIm]BF4 IL was used as a sensing interface to cross-link goat anti-humman immunoglobulin G(anti-IgG) via glutaraldehyde(GA) to fabricate an Bi2Se3/IL/GA/anti-IgG-CPE. The bovine serum albumin(BSA) was employed to enclose the residual nonspecific binding to further develop a label-free IgG immunosensor. The result showed the proposed label-free IgG immunosensor exhibited good performances toward IgG with detection limit of 0.8 ng mL-1 and linear ranges from 2 to 300, and 300 to 2200 ng mL-1. Besides, the immunosensor demonstrated nice specificity for IgG detection, acceptable reproducibility and stability. Thus, the Bi2Se3 material reported here paved a simple way to design a sensitive and cost-effective sensing platform for extension to other disease biomarkers.