| Objective In our study, in view of the amphiphilic of some natural products, we combined with a new technology(molecular imprinting technique, MIT), using the surface molecular imprinting strategy, to build molecular imprinted polymers(MIPNPs), which have high recognition and combination with the templates. The imprinting strategy has the advantage that, for the amphiphilic template molecules, the identifying sites are located in the surface of the prepared polymers, which could effectively avoid excessively embedding template molecule and improve its removal and so on, compared with the traditional imprinting method.Methods and results In the first part of the study, we obtained the polymer by inverse microemulsion polymerization, the template here was lipopolysaccharide, an amphiphilic molecular derived from pseudomonas aeruginosa. The prepared polymer was rounded in morphology, and had suitable sub- micrometer particle size with homogeneous distribution, the average particle size was(38.31 ± 2.13) nm, and the polydispersity index was(0.112 ± 0.102). Detected by fluorescence polarization technique, the MIPNPs showed a significantly high response to LPS, which was the same as observed by the MST assay. In vitro targeting ability measured by flow cytometry, indicated that the high affinity between the MIPNPs and LPS were primarily responsible for the observed selective recognition of the target specific bacteria. In addition, we established meningitis mice model, using an in vivo imaging system, we found that the levels of fluorescence intensity in the infected region(brain) were significantly higher, both in vivo and in vitro.Studies on the above shows that, for the amphiphilic template molecule, lipopolysaccharide, prepared imprinted polymers by inverse microemulsion polymerization, showed high affinity to template molecule both in vivo and in vitro. It turned out to showe that the preparation technology and research results had good feasibility and development foreground.In the second part, we studied ginseng saponin, which is also a kind of amphiphilic active ingredients. We choosed Rg5 to be the template, the MIPNPs were obtained by inverse microemulsion polymerization. The Rg5-MIPNPs also had suitable sub- micrometer particle size with homogeneous distribution. We determined the ability of imprinted polymers by high performance liquid chromatography(HPLC). The results showed that, Rg5-MIPNPs showed high affinity to template molecules. We also determined a variety of other ginseng saponin(R1) and glycyrrhizic acid(GA), results showed that the MIPNPs, with Rg5 as template molecules, showed specific binding abilities with Rg5. Based on this, we prepared gel column using the molecularly imprinted polymer. By studying the binding ability, We found that the gel column has good specificity recognition ability with Rg5 only.Conclusion In our study, we have presented a molecular imprinting, by inverse microemulsion polymerization, to improving the recognition of two kind s of natural amphiphilic components. On the one hand, using LPS as template, our study showed an attempt to design a magic bullet by molecular imprinting that may provide a novel approach to generate synthetic carrier for targeting pathogen and treatment for a variety of infectious human diseases. On the other hand, using ginseng saponin as template, to a certain extent, improve the separation and purification of active ingredients and promote the development of traditional Chinese medicine. |
