Impacts Of Alkyl Modified Chitosan On The Structure And Function Of Important Components In Blood

Chitosan is widely used in biomedicine due to its good biocompatibility, whereas limitations in application exist, which make chemical modification especially hydrophobic modification promising. In this study, hexanoic acid, decanoic acid and stearic acid were employed to modify the structure of chitosan in the EDC/NHS system to synthesis amphiphilic chitosan derivatives. The chemical structure of chitosan and its derivatives were measured by FT-IR and 1H NMR. Based on the synthetic route, chitosan derivatives with different degree of alkyl substitution, 5% and 10%, were prepared by changing the ratio of fatty acid. Then impacts of chitosan and its alkyl modified derivatives on the structure and function of important components in blood were studied to evaluate the hemocompatibility.Firstly, effects on proteins were analyzed, the secondary structure of BSA, γ-Globlulin and Fibrinogen were examined by UV absorption spectroscopy, fluorescence spectroscopy and circular dichroism, respectively. Secondly, red blood cells, platelets, coagulation and complement system were researched to estimate the blood compatibility of materials. At last,mouse monocyte-macrophages RAW 264.7 was introduced to investigate the impact of chitosan and alkyl modified chitosan derivatives on inflammatory, physiological activity and cytokine in detail. The study indicated that alkyl modified chitosan derivatives influence the structure and function of important components in blood, and different kind of derivatives have various impacts because of their differences in surface charge, hydrogen bond and hydrophobic force.