| Sea cucumber is an important marine invertebrate, and is easily be subjected to autolysis and vomiting intestine, which leads to severe deterioration in sea cucumber quality during handling and storage, which leads to huge economic losses.The effect of endogenous collagenase from S. japonicus guts on collagen degradation from body wall was investigated in this study. Firstly, to control the experiment temperature,the denaturation temperature of the body wall and crude collagen fiber(CCF) tested by differential scanning calorimeter. The optimal p H and temperature of endogenous collagenase from gutwas determined to be p H9 and 40℃ by gelatin zymography. Pepsin-soluble collagen(PSC) from body wall can be degraded effectively by endogenous collagenase. The band intensity of γ-chain, β-chain and α-chain was obviously decreased by treatment with endogenous collagenase for 0.5 h at p H9 and 40 ℃. TCA-soluble oligopeptide content was enhancedin this process. In addition, the rheological behavior of sea cucumber body wall crude collagen fiber(CCF) was analyzed by using rheometer. These results suggest that sea cucumber endogenous collagenase may be involved in collagen degradation.In addition, the rheological behavior of sea cucumber body wall crude collagen fiber(CCF) was analyzed by using rheometer. The elastic properties was higher than viscous properties of CCF at concentrations of 1%, 2 % and 3%. This result showed that the gel property of CCF was better at higher concentration. We also analyze the influence of the rotate speed to CCF, the results shows that the highest storage modulu is 1446.62±12.08 Pa in 2000 rpm. After digestion by sea cucumber endogenous collagenase, the liquidity of CCF was enhanced with decrease of gel property. These results suggest that sea cucumber endogenous collagenase may be involved in collagen degradation in the process of autolysis by influence the rheological behavior of sea cucumber body wall collagen fiber. |
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