Study Etuake Area Of Microbial Contamination Of Spirulina Powder

This test to Inner Mongolia Ordos Etuoke Banner Spirulina industry production base of spirulina powder samples, separation and control measures of harmful bacteria, microbial contamination were detected and analyzed。Firstly, from the morphological characteristics and physiological and biochemical aspects of isolated target bacteria were identified, after identification, Clostridium perfringens a total of 6 strains of bacteria, in different medium observed the growth characteristics of Clostridium perfringens, and research and to select the most suitable medium for Clostridium perfringens isolation and enumeration as the optimum culture medium. Secondly, the development of temperature and pH tolerance test for Clostridium perfringens, explore the spirulina powder physical prevention and control measures. The use of polylysine antibacterial test for Clostridium perfringens to determine the minimum inhibitory concentration of lysine amino acids in optimum pH. The test results showed that TSC medium is more favorable for the production of Clostridium perfringens in isolation and enumeration.40℃ for Clostridium perfringens optimal growth temperature, growth in the 15℃ to 65℃ DEG range; The 8.5 is the most suitable pH of Clostridium perfringens, not growth in addition to pH 5～10 range of Clostridium perfringens. At pH 7.5, poly lysine of Clostridium perfringens minimum inhibitory concentration was 0.025%, poly lysine can be used as one of the spirulina powder in the production of harmful bacteria inhibitor.The samples were counted, and the development temperature, acid and alkali tolerance tests were carried out. Test results show that:Coliform group 94/g,120/g, respectively, according to the national standard of spirulina powder coliform group shall not be greater than or equal to 90/g, coliform group testing unqualified. The optimum growth temperature of E. coli was 40 degrees Celsius, greater than 50℃ or less than 5℃, the optimum growth of pH was 8, and the pH was greater than 10.5 or less than 5.In accordance with the national standards for microbiological examination of food hygiene standard of spirulina powder of yeast count and the total number of colonies of determination, Salmonella enteritidis test, Staphylococcus aureus test, mold, Shigella determination. Experimental results show that Spirulina powder samples the total number of colonies were 4.6 x 104CFU/g,4.5 x 104CFU/g, is more than or equal to 1 x 104CFU/g standard.Mold inspection, did not detect the pathogenic bacteria Staphylococcus aureus, Salmonella, shigella.