Study On The Acid Stimulation Response Intelligent Nanocontainers And Properties For Controlled Release

Nanocontainers usually refer to nanoscale-composite materials, which can store and release the target substance. As is known to us, mesoporous silica nanoparticles microspheres (MSNs) have been widely used as ideal nanocontainers in the field of biological, medicine and chemical industry, because of their large capacity, ease surface modification and excellent biocompatibility. Especially the intelligent nanocontainers based on mesoporous silica nanometer, with a particular performance of stimulus-response, can absorb and release the target molecular through supramolecular nanovalve by external stimuli, such as pH, temperature and redox. Among these stimulis, pH has been researched widely as a external stimuli. In the paper, in order to construct an acid stimulation response intelligent nanocontainer drug carrier, we will do some researches on the preparation of acid stimulation response mesoporous silica nanoparticles microspheres (MSNs) and drug molecule’s controlled release, including the following aspects.The mesoporous silica nanoparticles microspheres (MSNs) were used as nanocontainers, which were prepared with Stober’s method, and firstly connected to seven C6s of amino -β-cyclodextrin with the help of the new material 2-(2-bromine-ethoxy)-2-(2-azide base-ethoxy) propane at the place of mesoporous to encapsulate Rhodamine B which was designed to simulate anti-cancer drugs in order to get the acid stimulation response intelligent nanocontainers with targeted anti-cancer effects. The morphology and structure of MSNs were characterized by scanning electron microscope/transmission electron microscope (SEM/TEM), X-ray diffraction assay (XRD), Brunauer-Emmett-Teller (BET) and Barrett-Joyner-Halenda (BJH) analyses. The functionalized surface of MSNs was demonstrated by Solid State Nuclear Magnetic Resonance Spectra (SS NMR), Fourier Transform Infrared Spectrometer (FTIR), Thermo Gravimetric Analysis (TGA) and so on. The releasing performances of Rhodamine B at different acidic pH values were monitored by Fluorescence Spectrophotometer (RF-5201) and the drugs loaded in the intelligent nano-container were final 15.23 mg/g which was measured by Ultraviolet-Visible Spectrophotometer (UV-Vis). The results showed that the intelligent nano-containers constructed by assembly-technology realized "zero release" under neutral condition and remarkable release under acidic condition-suitable release in imitation of the condition of Lysosome and Endosome as targets for anticancer, which has extensive application prospects in terms of targeting tumor cells. Moreover, the intelligent nano-containers can be fully used in nanocatalyst, anticorrosion coating and so on at the same time.