Fabrication And Application Based On Reduced Graphene Oxide Field Effect Biosensing Device

Field electric transistor (FET) biosensor, is one of the most potential candidates for the applications of biosensing, which can converted the biological signals into electrical signals directly, and can rely on the development of signal processing, integrated circuits and new materials to obtain a small size, high integration, high sensitive and real-time monitoring electronic devices with high integration for biology detection. Graphene, as a two dimensional material obtained from a single layer of carbon atoms which is closely packed, with a large surface area, high carrier mobility and has low noise. It is an ideal material for electrical biosensing. Currently, the method of graphite redox is used by more researchers, because it can regulated the conductive properties, oxygen-containing functional groups and surface defects. Due to it’s also high biological activities, it has a great interest and research value in the applications of biosensing.In this paper, the reduced graphene oxide film was prepared by chemical and thermal reduction treatment from graphene oxide film, which is self-assembled in our laboratory, (i) The r-GO as a sensing channel for field effect transistor device, the relevant electrical characteristics was studied. From the results, it was confirmed that the conductive properties of the r-GO was greatly improved about 106 times after reduced by ascorbic acid and heat treatment, (ii) In addition, the reduction degree of graphene oxide film based on the interdigital electrodes need to be improved to keep the stability of the Au/graphene interface in the solution, (iii) Furthermore, the changes in the electrical characteristics of FET device was analyzed and demonstrated the detection capability and mechanism of the modified by ConA from an detection of trypsin inhibitor, (iv) The electrical response of the device to colon cancer cells was also analyzed by the construction of cell-based detector, and the detection mechanism of the device based on cell activity was further analyzed.