| Acrylamide(AM), with many hazards to the huamn such as neurotoxicity, reproductive toxicity, genetic toxicity, has been listed as possible carcinogens by the International Agency for Research on Cancer. AM widely found in starchy foods after frying, baking and other high-temperature treatment, there is a huge threat to human health. Therefore, it is very important to establish a rapid, sensitive and suitable for field testing detection method for AM detection. Hemoglobin sensor, combine biosensor with electrochemical analysis, has been widely used in analytical testing because it has high sensitivity, good selectivity, fast response and other advantages. The porous carbon material have a large specific surface area, high conductivity, and excellent biocompatibility advantages can be well applied to build Hb sensor. Porous carbon has poor dispersion and water-soluble. In this study, the preparation of porous carbon immobilized ionic liquids, AuNPs-porous carbon immobilized ionic liquids, AuNPs-nitrogen-doped porous carbon nanomaterials to improve the performance of porous carbon. The Hb sensor, preparation use nanoparticles composite as carriers for the immobilization of Hb, applied to detection of AM. The results are as follows:1. In order to improve the dispersion and water soluble problem of porous carbon, combine porous carbon with ionic liquid 1-amino propyl-3-methylimidazolium bromide(IL-NH2) preparing porous carbon immobilized ionic liquid as Hb immobilized materials used to construct the Hb sensor. We used AC impedance to characterize different electrode, Porous carbon Supported Ionic Liquid/GC electrode and the bare GC electrode resistance values were 120 Ω, 50 Ω, respectively. It Shows that porous carbon immobilized ionic liquid reduced the interfacial resistance, increase the electron transfer rate. Hb loading, the reaction temperature and reaction time of AM and Hb were optimized. Hb loading On the sensor was 40 μg, the response signal of the sensor reach 9.87 μA, the optimum reaction temperature is 35 ℃. study the electrochemical behavior of acrylamide on the modified electrode. The adding of acrylamide and hemoglobin was proportional to its concentration ranging from 2×10-10 to 2×10-7 μmol/L and 2×10-7 to 7×10-5 μmol/L,with a detection limit of 1.40×10-11 μmol/L.2. To further improve the sensitivity of the experiment, gold grows on the surface of porous carbon immobilized by ionic liquid, and for the preparation of Nafion/AuNPs-porous carbon Supported Ionic Liquid-Hb/GC sensors. In this study, the method of fixing the Hb was optimized, response signal of the sensor can reach 9.87 μA. AM and Hb reaction time was optimized, and The optimum reaction time was 12 min. Studing in Nafion/AuNPs-porous carbon Supported by Ionic Liquid/GC sensor, from 7×10-11 mol/L to 7×10-9 mol/L and 7×10-9 mol/L to 7×10-5 mol/L, the addition rate of AM and Hb was linearly related to the negative logarithm of the concentration of AM with the detection limit of 3.83×10-12mol/L. According to detecting actual samples, the average recovery was 107.91%~115.97%.3. Using ionic liquid 1-butyl-Tri-methylimidazole dicyanamide salt(BMIMdca) as a precursor and silica balls as the hard template to prepare nitrogen-doped porous carbon, AuNPs was introduced on the nitrogen-doped porous carbon surface using chemical reduction method. And AuNPs-nitrogen-doped carbon nano-porous material was prepared as Hb’s fixed vector to prepare Nafion/AuNPs- nitrogen-doped porous carbon-Hb/GC sensor. We used Cyclic voltammetry to characterize different electrode, the bare GC electrode, nitrogen-doped Porous carbon Supported Ionic Liquid/GC electrode and AuNPs- nitrogen-doped porous carbon Supported by Ionic Liquid/GC Peak current values were 75.40μA,87.69μA, 93.00μA respectively.The amount of Nafion, nitrogen-doped porous carbon Au NPs-nano-materials consumption were optimized. Nafion loading on the sensor was 0.1%, The optimum amount of AuNPs- nitrogen-doped porous carbon nanomaterials was 3 μg. Under optimal conditions, the signal of Hb sensors can reach 12.50 μA.Under optimum conditions, The addition rate of AM and Hb was linearly related to the negative logarithm of the concentration of AM at the range from7.0×10-11 mol/L to 2.0×10-5 mol/L, and the detection limit is 1.81×10-13mol/L. Finally the actual sample is tested, and the recovery was 106.36~111.22%. |
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