Concentration And Distribution Features Of OCPs In Fishes Of Chao Lake

Organochlorine pesticides (OCPs) is recognized as environmental priority control pollutants. This kind of material is through the rain, surface runoff into aquatic ecosystems, after accumulating in the food chain, on the organism, teratogenic, carcinogenic and mutagenic "three" effect, which cause serious damage to aquatic life, health and safety of aquatic products. Fish at the top of the food chain of aquatic ecosystem, is one of the biggest species by OCPs harm. Fish OCPs enrichment characteristics research, is attention hot spot in recent years. In the early 1980 s in our country has been disabled for organochlorine pesticides. But because of organochlorine pesticides since 1957 decades of endless drop and its difficult degradation in the environment and strong the organochlorine pesticide chemicals that lead to large area of farmland use with rainfall and surface runoff way spread through the food chain in the biological accumulation in the body. Once organochlorine pesticides through various channels into the organism, in biological body fat, embryo, and accumulated in organs like the liver, accumulated to a certain extent will cause harm to the organism.Chao lake is one of the five largest fresh water lake in China, as well as Hefei Jianghuai region especially important eco-function areas. For a long time, large quantities of pesticide with rainfall and surface runoff converged in Chao lake, in the Chao lake ecological system migration, transformation and enrichment of wetland ecosystem health, causing serious pollution fishery production and the safety of drinking water. Moreover once lakes by organochlorine pesticide pollution will be across the lake ecosystem. In the aquatic food chain, fish represents the highest level of nutrients, certain pollutants of lower organisms may not cause significant change, but the fish may be affected. In addition with the improvement of living standards, food safety problems have been widely concerned by people more and more. Nowadays the fish enrichment of OCPs research mainly focused on the detection of the muscle tissue content and the corresponding risk assessment. And the enrichment characteristics in the related organizations haven’t got enough attention. The amount of organization of OCPs will get fish for our life enrichment of OCPs in the system.In order to understand the tissue accumulation of OCPs in five different diet fishes of Chao Lake, This article chose the five kinds of common Chao fish that silver carp, black carp, crucian carp, snakehead and catfish as the research object, and toprobe into the rationality of back muscle sampling method. Fish samples was salvage in Chao lake with fishermen in October 2013. We analyzed the concentrations of HCHs and DDTs in nine body parts of five fish species which from Chao Lake by the following methods of soxhlet extraction, sulfonate degrease, chromatographic column purification, rotary evaporation, nitrogen blow concentration, analysis by GC/MS. Freeze drying after 48 h 10 g fish sample 1-1 after blending with anhydrous sodium sulfate in 100 mll:1 ratio of soxhlet extraction 18 h in hexane and acetone solution. Extract by concentrated sulfuric acid sulfonated to fat, and then go to the sulfonated using 3% sodium sulfate solution. To sulfonated get samples of the fluid through the packing of alumina, silica gel glass column chromatography purification. Purification liquid by the rotary evaporation apparatus and the capacity to 1 ml after the blowing nitrogen use gas chromatography and mass spectrometry detector (GC/MS) analysis. All samples by adding the internal standard substance,4,5,6-2 tetrachloro meta-xylene (TCMX) out of the way to recovery range of 84.4% 103.1%. By external standard method to calculate their recoveries and relative standard deviation. Results show that the organochlorine pesticide recovery range of 81.74%-81.74%, relative standard deviation (RSD) is 3.307%-10.058%, meet the requirements of pesticide residue analysis. Recovery range to meet the requirements analysis. Discussed the OCPs in the distribution of differences between different trophic species and their distribution characteristics in the organization and corresponding consumption of risk assessment. Subject of the main conclusions are as follows:(1) Concentrations of OCPs in typical fishes of Chao Lake appear that carnivore fishes> phytophagous fishes> polyphagia fishes. Except the carnivorous snakehead and phytophagous carp difference was not remarkable (P= 0.088> 0.05, N=20) outside the rest between two species have significant difference (P < 0.010, N=20).(2) The organochlorine pesticides in the enrichment offish may mainly because of organochlorine pesticide residue after long-term degradation results. Chao lake region of DDT degradation of the environment is given priority to with aerobic environment, and no recent DDT input.(3) There is a significant positive correlation between fish tissue and the fat content (P<0.020, N=45), Fat content of tissue is one of the key factors for OCPs accumulation.(4) Fish between different tissue parts, enrichment of content in the intestinal digestive system is all organizations of the highest place, followed by liver metabolism system of the pancreas. Fish liver, pancreas and eggs (fine) nest enrichment of OCPs in the content is relatively higher than that of the content of muscle tissue, but the difference was not significant (P> 0.577, N=32). Brain tissue enrichment of OCPs content is higher than that of muscle tissue, brain tissue of the fish similar law of high concentration of heavy metal parts. Accumulations of OCPs appear that thatalimentary system>reproductive system> respiratory system.(5) The intestinal contents of OCPs is significantly higher than the gills. Furthermore, ingestion is an important way that could cause OCPs accumulation.(6) Catfish and snakehead tail muscle and silver carp and snakehead abdominal muscle OCPs content in the highest content of muscle tissue, and were significantly higher than the back muscles. The accumulation of back muscle is just one of the features of OCPs in entire fish muscles. Suggestions on the analysis of the content of fish muscle OCPs choose each mixing the muscle samples.(7) Estimation of organochlorine pesticide concentration of Chao Lake fish daily food intake is far lower than the national standard of daily food intake. Consumption of Chao Lake fish is a lower risk of organochlorine pesticide harm.