Development Of A Colloidal Gold-based Immunochromatographic Strip For Multianalysis Of Mycotoxin

In this study, it developed the detection of fumonisin B1, aflatoxin B1 single residue colloidal gold immunochromatographic test strip and the simultaneous determine-ation of two mycotoxins multi residue colloidal gold immunochromatographic test strip.By optimization, the best work condition of fumonisin B1 colloidal gold immunoch-romatographic strip is:colloidal gold particle size is 18 nm; the cellulose nitrate film (NC membrane) choose Millipore HF 135; colloidal gold labeled antibody protein, the optimal pH value is 7.5, the optimal amount of antibody is 30 μg/mL; the optimal amount of coating antigen is 0.07 g/strip, gold labeled antibody was diluted to 1:5; Sheep anti rabbit two anti dilution multiple of 1:150; coated diluent for the PB buffer solution; gold standard pad treatment solution for PBS buffer solution. Fumonisin B1 colloidal gold immune-chromatographic test strip for the visual detection limit was 5 ng/mL, the detection time is less than 10 min. There is no cross reaction with other mycotoxins, the method has good specificity. The detection limit is 100 ng/g in grain samples.Through optimization, the best work condition of aflatoxin B1 colloidal gold immunechromatographic strip is:colloidal gold particle size is 18 nm; the cellulose nitrate film choose Millipore HF 135; colloidal gold labeled antibody protein, the optimal pH value is 8, the optimal amount of antibody is 20 μg/mL; the optimal amount of coating antigen is 0.07 g/strip, gold labeled antibody was diluted to 1:5; Sheep anti rabbit two anti dilution multiple of 1:130; coated diluent for the PBS buffer solution; gold standard pad treatment solution for PBS buffer solution. Aflatoxin B1 colloidal gold immune-chromatographic test strip for the visual detection limit was 0.5 ng/mL. The detection time is less than 10 min. There is no cross reaction with other mycotoxins, the method has good specificity. The detection limit was 5 ng/g in grain samples.Through the optimization of fumonisin B1, aflatoxin B1 multi residue colloidal gold immunochromatographic test strip, determine the best working conditions is:fumon-isins gold labeled antibody and aflatoxin gold labeled antibody mixture ratio of 2:1; NC membrane underlined sequence as the control line, detection of FB1 line, AFT line detection; sample pad processing liquid for 0.25% Tween20 water solution, other working conditions are the same as the single residue work condition test strip. Fumonisin B1, aflatoxin B1 multi residue colloidal gold immunochromatographic test strip for visual detection limits were 5 ng/mL,0.5 ng/mL. The detection time is less than 10 min. The limit of detection of grain samples were 96 ng/g and 6 ng/g.