| Saccharomyces cerevisiae as a kind of industrial microorganisms, since it has short growth cycle and strong fermentation ability features, widely used in winemaking. The basic research of saccharomyces cerevisiae for human research to provide theoretical support for other higher eukaryotes.Aging is inevitable physiological phenomena to any creature, there were lower metabolism of sugar, higher flocculation, bacteria subsidence and decay, ultimately affect the yield and product quality in the aging S. cerevisiae FFC2144 yeast which were sub-cultivated continuously. Since Egilmez and Jazwinski put forward a hypothesis which the cytoplasm aging factors of Saccharomyces cerevisiae aging in 1989, Saccharomyces cerevisiae aging mechanism studies made great progress, set up replication senescence (replicative aging) and natural aging (chronological aging) two models. It have proved extrachromosomal rDNA ring (ERC) accumulation is the main reason for the beer yeast replication senescence. on account of the cell aging is caused by many factors, biological phenomena are complex, It happened inevitably involved in diverse, dynamic, woven into a network of proteomic performed by the result of the physiological function. In order to break the limitations of a single protein research, using two-dimensional electrophoresis analysiss the different generations of saccharomyces cerevisiae cell wall proteins group, laying a foundation for elaborate regulatory mechanism of yeast cell aging in the level of dynamic and systematical.In order to elucidate the regulatory mechanism of senescence in Saccharomyces cerevisiae FFC2144. The cell morphology, dynamic of growth rate, fermentablity of glucose, flocculation rates were monitored during the continuously subculturation of S. cerevisiae FFC2144. At the same time, the total proteins from differential generation of S. cerevisiae FFC2144 were extracted by phenol extraction method and dispersed with two-dimensional electrophoresis. The results showed that:there were higher flocculation, lower metabolism of sugar and cell surface were densely granulated in the aging S. cerevisiae FFC2144 yeast which were sub-cultivated continuously. Subsequently, we detected 3 spots de novo synthesized proteins and 2 up-regulated spots in the 2D image of 8th which compared with the 1th generation. Furthermore, found 3 down-regulated spots and 6 spots were disappeared in the 16th generation. Identification and proteomic analysis those total 14 proteins will promote better insight into the regulatory mechanism of the senescence of yeast.We use MALDI-TOF-MS to dentify the differential cell wall proteins.we focused theproteomic analyses on a number of specific proteins:pyruvate kinase,glyceraldehyde-3-phosphate dehydrogenase,transketolase (down-regulation);Heat shock protein,Flocculati on protein (de novo);Copper zinc superoxide dismutase,Sulfur redox proteins reductase (up-regulation);40s ribosomal protein (disappeared).It can be found that these prote ins involved in yeast energy metabolism,anti-aging repair mechanism and life cycle thr ough the analysis of the metabolic pathways of these differential proteins. |
