Research And Application Of Nano-Latex And Oligosaccharide Analogs In Capillary Electrophoresis

Capillary electrophoresis is analysis technology using dielectric migration of charged particles in an electric field for the separation of chemical or biochemical component. The capillary electrophoresis research focuses on tube-produce technology, the synthesis of system additive and innovation of electrophoretic pattern. In this paper, we have synthesized two kinds of Nanoparticles(NPs) and an oligosaccharide analogs. There will be applied in capillary electrophoresis tube filler and chiral additives, respectively. We have established a method for separation and analysis of biological samples, such as creatinine, phytohormones and oligosaccharide analogs. The specific content includes three parts as follows:1. Dimethylethanolamine amination polychloromethyl styrene nanolatex(DMEAPL) was been successfully synthesized, which as filler to the prepared DMEAPL coated capillary column(ccc-DMEAPL). A novel analysis method for creatinine in plasma was constructed by FASS-OT-CEC with ccc-DMEAPL. The optimized determination conditions were as following: ccc-DMEAPL; the coated capillary columns, 50 μm i.d.×50 cm(effective length 41.5 cm); background electrolyte, 30 mmol/L HAc+65 mmol/L NaAc(pH 4.97); UV detection wavelength, 205 nm; column temperature, 25 ℃;separation voltage, +20 kV; water-plug injection: 30 mbar×10 s; sample electrokinetic injection, +18 kV×22 s. Under the optimized conditions, the creatinine was analysized within 7 min, the detection limits were 10 ng/m L and the overall recoveries were more than 95%. The results demonstrate that the developed method is a efficient, rapid, accurate and sensitive method for the determination of creatioine in real samples.2. Methyldiethanolamine amination polychloromethyl styrene nanolatex(MDEAPL) was been successfully synthesized, which as filler to the prepared MDEAPL coated capillary column(ccc-MDEAPL). A novel analysis method for indole-3-acetic acid(IAA) and beta-indolebutyric acid(IBA) of different plants was constructed which combining tetraazacalix[2]arene[2]triazine-modiied silica gel(NCS) as solid-phase extraction(SPE) sorbent was developed for concentrating and purifying the two phytohormones by NCS-SPE-FASS-OT-CEC with ccc-MDEAPL. The optimized determination conditions were as following: ccc-MDEAPL; the coated capillary columns, 50 μm i.d.×50 cm(effective length 41.5 cm); background electrolyte, 30 mmol/L Tris+24 mmol/L HClO4(30% acetonitrile, pH 7.24); UV detection wavelength, 230 nm; column temperature, 25 ℃;separation voltage,-25 kV; water-plug injection: 30 mbar×20 s; sample electrokinetic injection,-20 kV×20 s. Under the optimized conditions which avoiding the introduction of an EOF reverse additive in buffer solution., the IAA and IBA were analysized within 7 min, the detection limits were 8-25 ng m L-1 and the overall recoveries were more than 81%. The results demonstrate that the developed method is a efficient, rapid, accurate and sensitive method for the determination of IAA and IBA in real samples.3. A water-soluble sulfonated oligosaccharide analogs was successlly synthesized. The chiral separation method of four kinds of citalopram enantiomers analogs was established using the oligosaccharide analog as chiral selector. Through the process for optimization of electrophoresis buffer and concentration, the concentration of chiral selector, the pH of buffer system, separation voltage and organic additive content, the separation of these four drugs optimized buffer system were obtained, which gave satisfactory results. Compared with the traditional method of cyclodextrin as chiral selector, it has the following advantages, such as the less amount of chiral selector, the higher resolution, the more universal, the simpler experimental methods and the greater maneuverability.