| There have been a large number of patients who suffered from the skin wound by different reasons in daily life. Wound dressings can effectively prevent the wound infection and secondary injury in the process of wound healing, the medical materials which were given to promote wound healing, become one of the most powerful measures in the process of wound healing. Electrospinning has been widely used to prepare biological nanofiber mats, the three-dimensional structures of which, mimic natural extra cellular matrix. The nanofiber mats have a series of performance of biocompatibility, non-toxicity, healing function, and can create a favorable environment of cell growth for the skin healing, which is one of ideal wound dressing.Ideal wound dressings must be given with the features not only biological characteristics, but also morphology characteristics, chemical properties, and mechanical properties. In current research, the properties of nanofibers are influeced by many factors, such as material type, solution concentration, flow rate, working distance, and voltage. However, only few studies focused on the effect of electrospinning techniques, on the properties of electrospun fibers.In this study, poly(L-lactide)(PLLA)/Zein nanofibers for new ideal wound dressing were prepared by different electrospining techniques, the main aim was to evaluate the effects of different electrospinning techniques. PLLA was fixed with zein load with rana chensinensis skin peptides(RCSPs), which were separated and extracted by Chinese Rana chensinensis, to evaluate the basic and biological properties.The PLLA/zein nanofiber mats prepared by blend, coaxial and double-nozzle electrospinning techniques, show smooth and complete apparent without the phenomemon of beads. The morphology characteristics, chemical properties, and mechanical properties of three nanofibers were influenced by the relation between PLLA component and zein component. This suggested that zein and PLLA were welldispersed without any chemical interactions in different electrospinning techniques,probably form newer hydrogen bonds. The blend fibers exhibited the lowest mechanical properties in the three techniques, probably because the uniform distribution of zein broke up the crystal growth structure of PLLA.In coaxial nanofibers, continuous PLLA core structure showed good mechanical performance.The pure PLLA fibers in double-nozzle nanofibers possessed very good mechanical properties.The PLLA/zein-RCSPs nanofiber mats prepared by blend, coaxial electrospinning techniques, show good morphology and larger nanofiber diameters than PLLA/zein nanofiber mats. It may due to RCSPs, which of small molecular weights,made the low viscosity and low electrostatic chargeof zein-RCSPs solution,leading higher diameters. In PLLA/zein-RCSPs nanofiber mats, the frequency of amide Ⅱ is shifted to lower regron. One possible cause for this shift is that the occurrence of intermolecular interactions between zein and RCSPs. The newer hydrogen bonds in turn affect the mechanical properties of PLLA/zein-RCSPs nanofiber mats. With adding RCSPs to zein solution, the composition of amino acids has changed to hydrophilic in a small range.We study the effect of RCSPs on the biological properties of nanofiber mats,research the adhesion and proliferation of HFF3T3-L1 onto PLLA/zein and PLLA/zein-RCSPs nanofiber mats after 5 days. more cells can be observed on the PLLA/zein-RCSPs nanofiber mats, and cell growth was significantly good. Among this system, compared with PLLA/zein nanofiber mats, PLLA/zein-RCSPs nanofiber mats, not only show good morphology characteristics, chemical properties, and mechanical properties, but also could provide better growth condition for cell proliferation, offering the most suitable qualification for skin wound dressing... |
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