Preparation And Characterization Of Hierarchical Hydroxyapatite And Calcium Carbonate

As a result of evolution for billions of years,Natural bone material has perfect structure and astonishing properties which people can’t imagine. However, its extensive clinical application has been hindered by the lack of mechanical properties. Through the extensive research of natural bone material, we learned that excellent biocompatibility and mechanical properties of natural bone materials largely due to multistage ordered assembled nanostructures. Therefore, microscopic structure assembly is the key to achieve the perfect combination of mechanical properties and biological properties in the preparation of artificial bone materials. Based on the basic concept of the structural bionics and functional bionics, summarize the results of the current study, multi-dimensional scaffold structure hydroxyapatite and lamellar calcium carbonate have been synthesized by a one-step hydrothermal reaction, to achieve increase in the mechanical and biological performance. By structure characterization(SEM, XRD), mechanical performance test(FMA), cell activity test(MTT, ALP) and other means to further study the structure and properties of materials. The main research contents and achievements in the paper were listed as follows:1. The multi-dimensional nanostructure hydroxyapatite were synthesized successfully by one-step hydrothermal reaction, which composed with highly ordered orthogonal intertwined nanorods. In the process, hydroxyapatite nanorods highly ordered self-assemble into the novel network like nanostructure, which is similar as a bed-mat for summer, and then the three-dimensional complex nanostructures were self-assembled from thin nanonets and nanorods induced by solvent system of structure-inducing agent. Furthermore, its biocompatibility was evaluated by culturing cells in vitro, the results show that multi-dimensional HA have more excellent mechanical properties compared with conventional HA nanorod. The mechanical properties of multi-dimensional HA was tested by three-dimensional nanorobot and Force Measurement Analysis(FMA) software, the results show that multi-dimensional HA have excellent mechanical properties. Which successfully solved the problem of insufficient mechanical properties of tissue filler material.2. The lamellar nanostructure calcium carbonate were synthesized by one-step hydrothermal reaction under the assistance of the structure-inducing agent. Furthermore, the thickness of the nanoplatelet more and more thin as the extension of reaction time. And we tested its biological activity and mechanical properties by culturing cells in vitro and three-dimensional robot respectively, the results show that the lamellar nanostructure calcium carbonate have good biocompatibility and excellent mechanical compression.Finally, this paper summarizes the specific work done, and proposed the problem to be further solved, pointed out the direction for further research.