The Research On The Reconstruction Technology Of Fish And Roe

In this paper, mackerel and herring roe were selected to be the subject of recombinant technique research of fish and roe. Through the research and investigation of traditional adhesive of food, the paper chose transglutaminase as the best adhesive. Optimize the processing conditions to make the adhesion of the products achieve a desirable level. It was proved that the processing conditions of producing the target recombinant products were feasible by the analysis of the texture and sensory evaluation. The SDS-polyacrylamide gel electrophoresis was used to analyze the change of the protein molecular mass of the recombinant products. The conclusions were as follows:1. In order to keep roe’s integrity during the drying process, we preferred the low-temperature heat-pump-drying technology by comparing different dehydration ways. The best drying parameters were as follows:1.5-2.0 h,35℃, and the final moisture content of the roe was 53.3%-61.8%.2. The article ultimately chose transglutaminase as the best adhesive of the reorganization of fish and roe through the research and investigation of the traditional adhesive. The final process flow was as follow:Check the raw materialsâ†' Pretreatmentâ†' Low-temperature heat-pump-dryingâ†' Addition of transglutaminase and excipientâ†' Moldâ†' Bonding reactionâ†' Sterilizationâ†' Packaged refrigeration3. Test the effects of all the factors those affect the adhesion of the products, and chose the cohesiveness as the main parameter to evaluate the products. The optimal processing conditions are as follows:addition of transglutaminase is 1.5% of the weight of roe, addition of soy protein isolate is 1.0%of that, the best time and temperature are 6 hours and 10℃.4. After the determination of the elasticity, hardness, color and volatile basic nitrogen of the recombinant products, it found that the texture and color of fish and roe have changed,but it was small and reasonable. Transglutaminase has great advantage in the adhesion of the products compared with the traditional adhesives.5. Transglutaminase influenced the recombinant products by catalyzing the cross-linking of most proteins. The SDS-polyacrylamide gel electrophoresis was used as the tool to prove the nature of the reaction. We found that the mass of proteins of the recombinant products becomes bigger as the addition of transglutaminase.