Study On Effect Of 4 Kingds Of Carrier Minerals On The Enrichment Culture Of Marine Nitrifying Bacteria

Recent years, with the development of economy and the market, aquaculture industrys get rapid development, marine aquaculture has become intensification, large-scale, highly productive mode development. C urrent the characterized by strong feeding way of factory farming increases the decomposition transformation load of organic nitrogen material in aquaculture water, the original microbes in aquaculture water cannot afford such a heavy burden, causeing water eutrophication and ecological imbalances, threating the survival of the breed.Current uses good effect, simple operation, no secondary pollution of b iological methods, namely, adding the nitrifying bacteria agents to remove the high concentration of ammonia nitrogen and nitrite nitrogen in the wastewater. Nitrifying bacteria is adherent microbe, need to grow in the carrier particles on the surface adsorption. N itrifying bacteria product is the high nitrification ability mixture of nitrifying bacteria who attached to the active carrier. N itrifying bacteria generation cycle, with slowly growth rate, easily affected by environmental factors, and the activity of the preservation hungry time is short. Therefore, improving the nitrification ability of nitrifying bacteria and longer preservation hungry time of nitrifying bacteria activity carrier is of great significance to the development of aquaculture.This experiment first uses Electron microscope scanning and particle size analyzer analyze the surface structure and particle size of four kinds mineral carrier(mineral-A, mineral-A, calcium carbonate, diatomite); And establish a kind of marine nitrifying bacteria enrichment culture device. Make a research on the influence of the four kinds mineral carrier nitrifying bacteria enrichment culture and nitrification intensity attenuation of nitrifying bacteria enrichment culture, through research get the following conclusion:(1) Four mineral carrier features vary, with most abundant mineral-D grain shape features, mainly for the disc-shaped, hole- like;Followed by mineral-A, mineral-B particles, primarily for shape representation of smooth and irregular granula r.Particle shape characterization of the rules is mineral-C particles, characterized by smooth-calcite. Moreover, the four species minerals carrier of grain diameter distribution range and the average grain diameter exists differences, mineral-A, mineral- B, mineral-C, mineral-D particle size range from 1.73~58.95μm、0.20 ~ 67.52 μm、, 67.52 ~ 67.52 μm、 1.98 ~ 67.52 μm, the average particle size were 12.98 μm、10.41 μm、16.08 μm、18.15 μm.(2) Establish a kind of simple structure, stable performance, simple culture of marine nitrifying bacteria enrichment culture device, establish a simple structure, stable performance, the culture system of this device is made up of aeration pump, agitator, intelligent temperature control instrument, culture vessel, dissolved oxygen and the p H meter constitute the monitoring system.(3) Different mineral carriers on marine nitrifying bacteria enrichment cultivation is different. Those marine nitrifying bacteria with mineral- D will get the strongest nitrifying capacity after enrichmentcultivation,its stable AOB and N OB average nitrification intensity of enrichment cultivation reach 297.59 mg N H4+-N/(L·d) and 243.05 mg NO2--N/(L·d), are respectively is 2.93 times and 2.93 times that of the blank control group, so the mineral-D is the best mineral carrier on promote nitrification intensity on marine nitrifying bacteria enrichment cultivation.(4) Best mineral carriers--mineral-D dosage proportion on marine nitrifying bacteria enrichment cultivation has a significant effect. marine AOB and NOB with different dosages conditionsof mineral-D(1%, 2%, 3%, and 4%), enrichment cultivation nitrification intensity get strength as the dosage increases, but the denitrification rate decreases as the dosage increases.(5) N itrification intensity on marine nitrifying bacteria enrichme nt cultivation adding carrier mineral with lengthen normal temperature preservation hungry time hungry its nitrification rate gradually decreases.Under the condition of normal temperature preservation hungry time hungry, adding the mineral-A, mineral-B, mineral-C, mineral- D carriers marine AO B enrichment cultivation prepared from the 3rd to the 7th month, nitrification intensity fell by63.9%, 64.2%, 58.9%, 66.6%; Under the condition of normal temperature preservation hungry time hungry, adding the mineral-A, mineral-B, mineral-C, mineral- D carriers marine NOB enrichment cultivation prepared from the 1th to the 5th month, nitrification intensity fell by 67.7%, 74.9%, 57.6%, 71.8%. And with the extension of preservation hungry time, nitrification intensity on marine nitrifying bacteria enrichment cultivation gradually slow down.