The Plant-Microbial Remediation Experimental Study Of Combined Pollution Soil With Oxytetracycline And Cadmium And Strengthen Measure

With the waste water irrigation and livestock manure entering soil, a great quantity of antibiotics and heavy metals compresence and effect each other in soil, threaten environment and human heath. The remediation for antibiotics and heavy metals pollution become more imminent. For the problems of the plant- microbial remediation of combined pollution soil with antibiotics and heavy metals, the study choose oxytetracycline and cadmium as target pollutants, Mirabilis jalapa L. and Tagetes patula L. as remediation plants, and add oxytetracycline high efficient degradation microbes, develop the plant- microbial remediation experimental study of combined pollution soil with oxytetracycline and cadmium, assisted with some strengthen measure of fertilizer, chelating agent and organic acid, look for one high remediation efficiency, improving soil ecological environment, lowcost method to manage this kind of pollution soil. The research results is as followed.1. selection two strains of oxytetracycline high efficient degradation microbes from sample of poultry dung compost, identify Phyllobacterium-myrsinacearum and Rhodotorula mucilaginosa. The two strains microbes were single and mixed management, all have degradation effect for oxytetracycline. Phyllobacterium-myrsinacearum degradation rate is 58.9%, Rhodotorula mucilaginosa degradation rate is 62.9% and mixed microbes degradation rate is 71.1%. The best degradation time is 8-12 h, the best inoculum concentration is 10 m L.2. Oxytetracycline and Cd both can restrain plants’ biomass and the biomass decline with the increasing of pollutant concentration. The No.11 and No.23 treatments with the highest pollution, not adding degradation microbes have least biomass, and the total dry weights are 3.087 g, 3.086 g. Adding degradation microbes can improve biomass obvious.The enrichment of Cd of plants declines with the increasing of the pollutant concentration. Adding degradation microbes can increase the Cd accumulation and Cd enrichment coefficent of plant. No.22 treatment has the highest enrichment coefficient, it is 4.30, the total accumulation is 108.259mg/kg, it has exceeded the critical value of hyperaccumulation plant. degradation microbes can degrade oxytetracycline. When the oxytetracycline is 5 mg/kg, the highest degradation rate is 70.1%, When the oxytetracycline is 30 mg/kg, the highest degradation rate is 59.3%. Compared the ability of enrichment Cd and degrading oxytetracycline, Tagetes patula L. is better than Mirabilis jalapa L..3. Fertilizer can improve the biomass. The effect of(NH4)2SO4 is best and the total dry weight is 4.940 g. Chelating agent decline the biomass. EDTA treatment’s biomass is lowest and the total weight is 3.614 g. Organic acid promotes plant to grow, but the effectiveness is not obvious. For total Cd accumulation, 8 strengthen measures ability are(NH4)2SO4≈KCl>EDTA>C4H6O6>H2C2O4>Ca(H2PO4)2>NTA>C6H8O7>CK. The effect of(NH4)2SO4 and KCl are the best, total accumulation are 93.939mg/kg and 91.209mg/kg。Fertilizer can improve oxytetracycline degradation rate and the degradation rate of(NH4)2SO4 is best which is 17.14%. Chelating agent decline the degradation rate and the degradation rate of NTA treatment is only 47.46%. C4H6O6 and C6H8O7 can improve the degradation rate obvious and their degradation rates are 60.55%、65.99%. The effect of H2C2O4 is not obvious. So(NH4)2SO4 is the best strengthen treatment.