Extraction,separation,purification,structure Analysis And Biological Activity Of Tremella Fuciformis Polysaccharide

Tremella fuciformis berk is one of the important members in the family of edible fungus, because of its high quality and inexpensive as the main window of edible fungi polysaccharide.(1)According to the response surface analysis and orthogonal experiment, the best extraction technology was obtained that the ratio of complex enzyme for cellulase: Papain: pectinase was 15:25:11; The Liquid to solid ratio was 80:1, ultrasonic power was 480 W, ultrasonic time was 40 min, and the content of the compound enzyme was 2%; Compared to four kinds of methods which were water extraction method, complex enzyme method, ultrasonic method and ultrasonic assisted composite enzymatic method, respectively extracate the Tremella Polysaccharide’s physicochemical index and antioxidant capacity, the four methods all showed good biological activity. The structure of TFP was not changed by IR analysis.(2) Three components of TFP30, TFP80, TFP60 were obtained by water soluble alcohol precipitation, the total sugar content of three groups were 40.32%, 45.31%, 72.13%; The antioxidant activity of TFP- water, TFP30 and TFP60 were studied. The results showed that the three kinds of fractions had good antioxidant activity. Through comprehensive analysis of each component, the TFP60 component was selected for the further purification.(3) The separation and purification of TFP60 components and using UV, IR and HPLC analysis to obtain the pure TFP60-S component, the molecular weight is 5.6 ×10~5 Da.(4) Using monosaccharide component、IR and GC-MS analysis the structure of TFP60-S,the result showed it was acidic and homogeneous heteropolysaccharide mainlycomposed of four monosaccharide residues, the anomeric carbons were α-configuration and containing O-acetyl groups. Acetylation analysis by GC-MS peak area indication that TFP60-S was composed of D-Mannose, D-Xylose, L-Fucose and D-Glucose,the molar ratio of 3.03:1:0.78:0.46.(5) The anti-tumor activity of TFP30,TFP60,TFP80,TFP60-S was investigated based on the test of A549 and CHL cells proliferation in vitro by MTT assay,The results showed that TFP60-S had good inhibition of tumor cell activity and had no toxic side effect was observed on normal cells.