Fabrication Of Micropatterned TiO₂ Nanotube On Ti Nanorods And Its Performance

Recent years the fabrication of Ti-based nanostructure as orthopedic and dental implant have been widely researched due to excellent its performance. At the same time micropattern technology have been introduced to Biomedical fields due to the understanding of vivo microenvironment such as tring to build a bionic microenvironment similar to vivo microenvironment by Micro/nano manufacturing technology and bionics principle. Different type nanostructure have different properties so there should be several kinds nanostructure on medical Ti materials to satisfy bone regeneration’s need. Thus micropattern materials technology have big potential in medical Ti materials’ modification fields according to its property.In this paper, controllable micropatterned TiO2 nanotube arrays are fabricated on Ti nanorods surface via combination of photolithography and electrochemical anodization, by which micro- and nano-scales geometry control can be obtained. At first Ti nanorods were fabricated on Ti surface through electrochemical anodization and then strip patterns of which width(or inner diameter) and separation distance are 200μm and 200μm respectively were established via photolithography on Ti nanorods surface. At last the TiO2 nanotubes were fabricated on the exposed portions through electrochemical anodization. Scanning electron microscope(SEM) were used to examine the quantity and morphology of nano- and microscales geometric patterns. The results show that anodization parameters, anodization voltage affect the size of TiO2 nanotubes arrays, and the pH of electrolyte affect the morphology of TiO2 nanotubes. Distortion of patterns could be severe when voltage is 5v, pH of electrolyte is 3 and oxidation Time is 30 min.To explore the surface wettability’s influence of TiO2 nanotubes and Ti nanorods on their protein absorption ability, we use FAS and oxygen plasma as modification method. Then Bovine Serum labeled by Cy3(BSA-Cy3) were chosed to explore protein adsorption and desorption behavior of TiO2 nanotubes and Ti nanorods under the same condition. Fluorescent inverted microscope and ImageJ were applied for qualitative and semiquantitative determination of protein adsorption and desorption respectively. Results indicated that proteins were much more preffer Ti than super-hydrophobic TiO2 nanotubes and Ti nanorods. Besides, the protein desorption speed and quantity of Ti nanorods are higher than TiO2 nanotubes.To explore the surface wettability’s influence of TiO2 nanotubes and Ti nanorods on their protein absorption ability, we use FAS and oxygen plasma as modification method. Then Bovine Serum labeled by Cy3(BSA-Cy3) were chosed to explore protein adsorption and desorption behavior of TiO2 nanotubes and Ti nanorods under the same condition. Fluorescent inverted microscope and ImageJ were applied for qualitative and semiquantitative determination of protein adsorption and desorption respectively. Results indicated that proteins were much more preffer Ti than super-hydrophobic TiO2 nanotubes and Ti nanorods. Besides, the protein desorption speed and quantity of Ti nanorods are higher than TiO2 nanotubes.The bioactivity of TiO2 nanotubes and Ti nanorods was examined by soaking them in the simulated body fluid(5 SBF). The results showed that TiO2 nanaotubes’ mineralization speed is fast than Ti nanorods at the nucleate stage. But the growth speed of Ti nanorods’ crystal nucleus is faster. Besides the mineralization materials of Ti nanorods will cover TiO2 nanotubes on micropatterned TiO2 nanotubes on Ti nanorods materials.